Larsen Pamela L
Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.
Sci Aging Knowledge Environ. 2003 Apr 30;2003(17):PE9. doi: 10.1126/sageke.2003.17.pe9.
Several genes involved in the determination of life span have been identified by mutation in the free-living soil nematode Caenorhabditis elegans. One of the key pathways studied in the context of life span is the DAF-2 pathway. The daf-2 gene is homologous to the insulin and insulin-like growth factor 1 receptor families. A downstream gene, daf-16, encodes a protein that is homologous to the forkhead transcription factor. A study by McElwee, Bubb, and Thomas, published in the current issue of Aging Cell, used genome-scale gene expression analysis to search for genes that are differentially expressed between long-lived daf-2(e1370) and short-lived daf-16(m27);daf-2(e1370) animals. In doing so, they identified candidate direct and indirect targets of DAF-16. In this Perspective, I discuss the results of this study.
通过对自由生活的土壤线虫秀丽隐杆线虫进行突变，已经鉴定出了几个与寿命决定相关的基因。在寿命研究背景下所研究的关键途径之一是DAF-2途径。daf-2基因与胰岛素和胰岛素样生长因子1受体家族同源。一个下游基因daf-16编码一种与叉头转录因子同源的蛋白质。McElwee、Bubb和Thomas发表在本期《衰老细胞》上的一项研究，利用基因组规模的基因表达分析来寻找在长寿的daf-2(e1370)和短寿的daf-16(m27);daf-2(e1370)动物之间差异表达的基因。通过这样做，他们鉴定出了DAF-16的候选直接和间接靶标。在这篇观点文章中，我讨论了这项研究的结果。
