Stroehlein Andreas J, Young Neil D, Hall Ross S, Korhonen Pasi K, Hofmann Andreas, Sternberg Paul W, Jabbar Abdul, Gasser Robin B
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, Australia.
Structural Chemistry Program, Eskitis Institute, Griffith University, Brisbane, Australia.
Parasit Vectors. 2016 Jun 24;9(1):360. doi: 10.1186/s13071-016-1642-y.
Proteins of the cysteine-rich secretory proteins, antigen 5 and pathogenesis-related 1 (CAP) superfamily are recognized or proposed to play roles in parasite development and reproduction, and in modulating host immune attack and infection processes. However, little is known about these proteins for most parasites.
In the present study, we explored CAP proteins of Toxocara canis, a socioeconomically important zoonotic roundworm. To do this, we mined and curated transcriptomic and genomic data, predicted and curated full-length protein sequences (n = 28), conducted analyses of these data and studied the transcription of respective genes in different developmental stages of T. canis. In addition, based on information available for Caenorhabditis elegans, we inferred that selected genes (including lon-1, vap-1, vap-2, scl-1, scl-8 and scl-11 orthologs) of T. canis and their interaction partners likely play central roles in this parasite's development and/or reproduction via TGF-beta and/or insulin-like signaling pathways, or via host interactions.
In conclusion, this study could provide a foundation to guide future studies of CAP proteins of T. canis and related parasites, and might assist in finding new interventions against diseases caused by these parasites.
富含半胱氨酸的分泌蛋白、抗原5和病程相关蛋白1（CAP）超家族的蛋白质被认为或被提议在寄生虫的发育和繁殖以及调节宿主免疫攻击和感染过程中发挥作用。然而，对于大多数寄生虫来说，人们对这些蛋白质知之甚少。
在本研究中，我们探索了犬弓首蛔虫的CAP蛋白，犬弓首蛔虫是一种具有重要社会经济意义的人畜共患线虫。为此，我们挖掘和整理了转录组和基因组数据，预测并整理了全长蛋白质序列（n = 28），对这些数据进行了分析，并研究了犬弓首蛔虫不同发育阶段各基因的转录情况。此外，基于秀丽隐杆线虫的现有信息，我们推断犬弓首蛔虫的选定基因（包括lon-1、vap-1、vap-2、scl-1、scl-8和scl-11直系同源基因）及其相互作用伙伴可能通过转化生长因子-β和/或胰岛素样信号通路，或通过与宿主的相互作用，在这种寄生虫的发育和/或繁殖中发挥核心作用。
总之，本研究可为指导未来对犬弓首蛔虫及相关寄生虫的CAP蛋白研究提供基础，并可能有助于找到针对这些寄生虫引起的疾病的新干预措施。
