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Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells.

作者信息

Dorin J R, Dickinson P, Emslie E, Clarke A R, Dobbie L, Hooper M L, Halford S, Wainwright B J, Porteous D J

机构信息

MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.

出版信息

Transgenic Res. 1992 Mar;1(2):101-5. doi: 10.1007/BF02513027.

DOI:10.1007/BF02513027
PMID:1284480
Abstract

We wish to construct a mouse model for the human inherited disease cystic fibrosis. We describe here the successful targeting in embryonal stem cells of the murine homologue (Cftr) of the cystic fibrosis transmembrane conductance regulator gene, as the first critical step towards this end. The targeting event precisely disrupts exon 10, the site of the major mutation in patients with cystic fibrosis. The targeted cells are pluripotent and competent to form chimaeras.

摘要

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Successful targeting of the mouse cystic fibrosis transmembrane conductance regulator gene in embryonal stem cells.
Transgenic Res. 1992 Mar;1(2):101-5. doi: 10.1007/BF02513027.
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