Mason H D, Willis D, Holly J M, Cwyfan-Hughes S C, Seppala M, Franks S
Department of Obstetrics and Gynaecology, Imperial College of Science, Technology and Medicine, St Mary's Hospital Medical School, London, UK.
Mol Cell Endocrinol. 1992 Nov;89(1-2):R1-4. doi: 10.1016/0303-7207(92)90224-t.
The effects of insulin-like growth factor-binding proteins (IGFBPs) 1 and 3 on steroidogenesis by human granulosa cells has been examined. Both IGFBP-1 and IGFBP-3 produced a dose-related inhibition of IGF-I-stimulated oestradiol accumulation in granulosa cell-conditioned medium with complete reversal of the effects of IGF-I in the presence of a molar excess of binding protein. IGFBPs 1 and 3 also exerted a small (25-40%) but significant and consistent inhibition of oestradiol secretion in response to follicle-stimulating hormone (FSH) alone. The progesterone response to IGF-I was inhibited by IGFBPs 1 and 3 but there was no effect on FSH-stimulated progesterone production. These data support the concept of a physiologically important intraovarian IGF system in the human ovary and demonstrate an unequivocally inhibitory effect of IGFBPs 1 and 3 on IGF-I-stimulated granulosa cell steroidogenesis.
研究了胰岛素样生长因子结合蛋白（IGFBPs）1和3对人颗粒细胞类固醇生成的影响。IGFBP-1和IGFBP-3均对颗粒细胞条件培养基中IGF-I刺激的雌二醇积累产生剂量相关的抑制作用，在存在摩尔过量结合蛋白的情况下，IGF-I的作用完全逆转。IGFBPs 1和3对单独卵泡刺激素（FSH）刺激的雌二醇分泌也有轻微（25%-40%）但显著且持续的抑制作用。IGFBPs 1和3抑制了对IGF-I的孕酮反应，但对FSH刺激的孕酮生成没有影响。这些数据支持了人卵巢中存在生理上重要的卵巢内IGF系统这一概念，并明确证明了IGFBPs 1和3对IGF-I刺激的颗粒细胞类固醇生成具有抑制作用。
