Ovesen P, Ingerslev H J, Orskov H, Ledet T
University Department of Gynaecology and Obstetrics, Aarhus Kommunehospital, Denmark.
J Endocrinol. 1994 Feb;140(2):313-9. doi: 10.1677/joe.0.1400313.
Numerous clinical and experimental observations have suggested that GH is important in ovarian function. We have investigated the effect of GH alone and GH in combination with FSH on the secretion of oestradiol, progesterone, insulin-like growth factor-I (IGF-I) and IGF-binding protein-1 (IGFBP-1) and on [3H]thymidine incorporation in cultured human luteinized granulosa cells. Granulosa cells from patients undergoing treatment for in vitro fertilization were isolated and cultured for 2 days in culture medium with 10% serum. After this preincubation, the medium was removed and the cells were incubated with GH (1, 10 and 100 micrograms/l) with or without FSH in serum-free medium and in the presence of [3H]methylthymidine (2 microCi/ml). GH alone resulted in a significant dose-dependent increase of oestradiol (P < 0.05) and in IGFBP-1 (P < 0.002) in the medium. The release of IGF-I was undetectable and there was no increase in [3H]thymidine incorporation with GH alone. Neither GH nor FSH alone stimulated granulosa cell proliferation or progesterone release, while the combination induced increases (P < 0.001) in both. The stimulatory effect of GH on steroidogenesis, IGFBP-1 production and granulosa cell proliferation supports a putative role for GH in the regulation of ovarian function.
大量临床和实验观察表明，生长激素（GH）对卵巢功能很重要。我们研究了单独使用GH以及GH与促卵泡激素（FSH）联合使用对雌二醇、孕酮、胰岛素样生长因子-I（IGF-I）和IGF结合蛋白-1（IGFBP-1）分泌的影响，以及对培养的人黄体化颗粒细胞中[3H]胸腺嘧啶核苷掺入的影响。从接受体外受精治疗的患者中分离出颗粒细胞，并在含10%血清的培养基中培养2天。预孵育后，去除培养基，将细胞在无血清培养基中与GH（1、10和100微克/升）一起孵育，有无FSH均可，同时存在[3H]甲基胸腺嘧啶核苷（2微居里/毫升）。单独使用GH导致培养基中雌二醇（P < 0.05）和IGFBP-1（P < 0.002）显著呈剂量依赖性增加。未检测到IGF-I的释放，单独使用GH时[3H]胸腺嘧啶核苷掺入也没有增加。单独使用GH或FSH均未刺激颗粒细胞增殖或孕酮释放，而两者联合使用则诱导两者均增加（P < 0.001）。GH对类固醇生成、IGFBP-1产生和颗粒细胞增殖的刺激作用支持了GH在调节卵巢功能中的假定作用。
