Akat Kemal, Mennel Hans-Dieter, Kremer Paul, Gassler Nikolaus, Bleck Christopher K E, Kartenbeck Jürgen
Division of Cell Biology, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120, Heidelberg, Germany.
Acta Neuropathol. 2003 Oct;106(4):337-47. doi: 10.1007/s00401-003-0736-8. Epub 2003 Jul 5.
Intercellular junctions morphologically identical to epithelial desmosomes are known structures in meningiomas and arachnoidal tissue. Desmoplakin as one of the desmosomal plaque components has proven to be a reliable marker for diagnosis of meningeal tumors. Here we demonstrate by immunofluorescence microscopy, immunoblot and reverse transcription-PCR reactions that cells of arachnoidal tissue, of diverse meningioma subtypes and of a meningioma-derived cell line contain the full complement of the typical desmosomal proteins desmoplakin (DP), plakophilin 2 (PP2), desmocollin 2 (Dsc2) and desmoglein 2 (Dsg2). Consequently, all these molecules are suitable for diagnostic applications of meningioma tumors. In addition to these constitutive desmosomal components, representative for single-layered (simple) epithelia, the dural border cells of the arachnoid and about 60% of the meningiomas tested were positive for desmocollin 3 (Dsc3), a protein in epithelia taken as an indicator for differentiation.
在形态学上与上皮桥粒相同的细胞间连接是脑膜瘤和蛛网膜组织中已知的结构。桥粒斑蛋白作为桥粒斑的组成成分之一，已被证明是诊断脑膜瘤的可靠标志物。在此，我们通过免疫荧光显微镜、免疫印迹和逆转录聚合酶链反应证明，蛛网膜组织细胞、不同脑膜瘤亚型细胞以及一种脑膜瘤来源的细胞系均含有典型桥粒蛋白桥粒斑蛋白（DP）、桥粒芯蛋白2（PP2）、桥粒黏附分子2（Dsc2）和桥粒芯糖蛋白2（Dsg2）的完整组合。因此，所有这些分子都适用于脑膜瘤肿瘤的诊断应用。除了这些代表单层（简单）上皮的组成性桥粒成分外，蛛网膜的硬脑膜边界细胞以及约60%的被测脑膜瘤对桥粒黏附分子3（Dsc3）呈阳性，Dsc3是上皮中的一种蛋白质，被视为分化的指标。
