von Eisenhart-Rothe B, Dominka T, Rödiger W, Ganschow I, Ivens B, Reckhaus M L, Renner V, Wirsig H W
Abteilung für Transfusionsmedizin und Transplantationsimmunologie, Chirurgische Universitätsklinik, Hamburg-Eppendorf, BRD.
Beitr Infusionsther. 1992;30:136-9.
In order to estimate the leftward shift of the oxygen dissociation curve of hemoglobin following storage of red blood cells (rbc) in the additive solutions SAG-M and PAGGS-M, respectively, we performed blood gas analyses after equilibrating the cells with a gas mixture containing 4% of O2 and 5% of CO2 at pH 7.4 and 37 degrees C. Additionally, we took advantage of these nearly physiological conditions to measure the recovery of the hemoglobin function in vitro. We observed a good correlation between 2,3-BPG and p50 (i.e. the oxygen tension, at which hemoglobin is half-saturated with oxygen). Within the first 3 weeks of storage, the 2,3-BPG content fell to one fifth of its original value, whereas the p50 declined from 26.6 to about 20 mm Hg. Compared to fresh cells, rbc stored for longer than 3 weeks will thus deliver 30% less oxygen to the myocardium. The rbc's ability to restore these parameters remained unchanged throughout 7 weeks of storage.
为了分别评估红细胞（RBC）在添加剂溶液SAG - M和PAGGS - M中储存后血红蛋白氧解离曲线的左移情况，我们在37℃、pH 7.4条件下，用含4%氧气和5%二氧化碳的混合气体平衡细胞后进行了血气分析。此外，我们利用这些接近生理的条件在体外测量血红蛋白功能的恢复情况。我们观察到2,3 - 二磷酸甘油酸（2,3 - BPG）与p50（即血红蛋白氧饱和度为50%时的氧分压）之间存在良好的相关性。在储存的前3周内，2,3 - BPG含量降至其初始值的五分之一，而p50从26.6 mmHg降至约20 mmHg。因此，与新鲜细胞相比，储存超过3周的红细胞向心肌输送的氧气将减少30%。在7周的储存期内，红细胞恢复这些参数的能力保持不变。
