Neppert J, Spindler J
Institut für Transfusionsmedizin im Klinikum, Christian-Albrechts-Universität, Kiel, BRD.
Beitr Infusionsther. 1992;30:66-9.
In order to improve the alanine aminotransferase (ALT) assay by using microtiter plates, a new 96-channel multiple syringe of high precision and incubation chambers with precise regulation of the temperature operated by computer were developed. In accordance with the recommendation of the German Society of Clinical Chemistry for the optimized standard method, the ALT assay was modified for the procedure at 25 degrees C. The modification was comparable with the best conventional methods which, however, are very time-consuming. The ALT assay has the advantages of the microtiter plate, e.g., secure sample identification, on-line documentation and reduction of costs and time for the analysis.
为了利用微量滴定板改进丙氨酸氨基转移酶(ALT)检测方法,研发了一种新型的高精度96通道多注射器以及由计算机操作精确调节温度的孵育室。根据德国临床化学学会对优化标准方法的建议,对ALT检测方法进行了修改,以适用于25摄氏度的检测程序。这种修改后的方法与最佳传统方法相当,但传统方法非常耗时。ALT检测方法具有微量滴定板的优点,例如,样品识别安全、在线记录以及降低分析成本和时间。