Burin des Roziers N, Pouilles F, Roche M, Claret G
Centre Départemental de Transfusion Sanguine, Centre Hospitalier Regional et Universitaire, Nîmes, France.
Transfusion. 1995 Apr;35(4):331-4. doi: 10.1046/j.1537-2995.1995.35495216083.x.
Alanine aminotransferase (ALT) testing in blood donors is required in many countries as a surrogate test for viral hepatitis. Current automated ALT assays do not fulfill some operational requirements of blood banks.
The standard ALT measurement method of the International Federation of Clinical Chemistry was adapted to the 96-well microtiter plate to obtain optimal conditions. The method was compared with a reference biochemical method using an analyzer on 251 serum samples.
The analytical variability was minimal (2.3% for an ALT level of 59 IU/L and 3.6% for an ALT level of 33 IU/L) after a total analysis time of 8 minutes and a time of 7 minutes before the first reading. With these measures, a linear standard curve was obtained in the range of 10 to 130 IU per L.A computerized validation procedure allowed rejection of samples with high ALT levels. Comparison with the analyzer gave a correlation coefficient, r = 0.935. The method proved to be efficient and time-saving in routine use over an 8-month period.
The microtiter plate ALT assay is helpful in allowing the simultaneous distribution of ALT and immunoenzymatic assays. It provides accurate results and a minimal risk of false-negative results.
在许多国家，对献血者进行丙氨酸氨基转移酶（ALT）检测作为病毒性肝炎的替代检测方法。目前的自动化ALT检测方法不能满足血库的一些操作要求。
将国际临床化学联合会的标准ALT测量方法应用于96孔微量滴定板以获得最佳条件。使用分析仪在251份血清样本上，将该方法与参考生化方法进行比较。
经过8分钟的总分析时间以及首次读数前7分钟的时间后，分析变异性最小（ALT水平为59 IU/L时为2.3%，ALT水平为33 IU/L时为3.6%）。通过这些措施，在每升10至130 IU的范围内获得了线性标准曲线。计算机化验证程序允许拒收ALT水平高的样本。与分析仪比较得到的相关系数r = 0.935。在8个月的常规使用中，该方法被证明是高效且省时的。
微量滴定板ALT检测有助于同时开展ALT和免疫酶检测。它能提供准确结果且假阴性结果风险极小。
