[基于重组梅毒螺旋体抗原的酶联免疫吸附测定和快速血浆反应素试验在梅毒诊断中的敏感性和特异性：一项比较研究]
[Sensitivity and specificity of ELISA based on recombinant Treponema pallidum antigen and rapid plasma reagin test in diagnosis of syphilis: a comparative study].
作者信息
Wang Lin-na, Zheng He-yi, Li Jun, Wang Xiao-feng, Liu Xiu-rong
机构信息
Department of Dermatology, Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100730, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2007 Jun 26;87(24):1721-2.
OBJECTIVE
To compare the sensitivity and specificity between ELISA based on the recombinant Treponema pallidum (Tp) antigen and rapid plasma reagin (RPR) test diagnosis of syphilis.
METHODS
Peripheral blood samples were collected from 249 patients, 177 males and 72 females, aged 34. Abbott Determine Syphilis TP test (ELISA based on the recombinant Tp antigen), RPR test, and Treponema pallidum haemagglutination assay (TPHA) were conducted with TPHA used as the gold standard.
RESULTS
The serum sensitivity and specificity of Abbott Determine Syphilis TP test were 100% and 98.4% respectively. The serum sensitivity and specificity of RPR test were 65.1% and 98.4% respectively.
CONCLUSION
The sensitivity of the rapid ELISA based on recombinant Tp antigen is significantly higher than that of RPR test.
目的
比较基于重组梅毒螺旋体（Tp）抗原的酶联免疫吸附测定（ELISA）和快速血浆反应素环状卡片试验（RPR）诊断梅毒的敏感性和特异性。
方法
收集249例患者的外周血样本，其中男性177例，女性72例，年龄34岁。采用梅毒螺旋体血凝试验（TPHA）作为金标准，进行雅培梅毒螺旋体抗体诊断试剂盒检测（基于重组Tp抗原的ELISA）、RPR试验和TPHA检测。
结果
雅培梅毒螺旋体抗体诊断试剂盒检测的血清敏感性和特异性分别为100%和98.4%。RPR试验的血清敏感性和特异性分别为65.1%和98.4%。
结论
基于重组Tp抗原的快速ELISA敏感性显著高于RPR试验。
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