Zhang M, Dai B
Hua Xi Yi Ke Da Xue Xue Bao. 1992 Sep;23(4):349-52.
Hebdomadis is one of the major serogroups found in China. In Sichuan, this serogroup appears to have a close relationship with local outbreak of leptospirosis. BALB/c mice were immunized intrasplenically with outer envelope of serogroup Hendomadis serovar hebdomadis strain 245. Spleen cells were fused with SP2/0 myeloma cells, two monoclonal antibodies Af2 and Bb2 were produced by hybridoma technique. McAb Af2 and McAb Bb2 were identified to be IgG1 and IgG3 by immunodiffusion respectively. Specificities of these two McAbs were determined by MAT; both reacted to 8 serovars (hebdomadis, nona, kambale, kremastos, worsfoldi, jules, maruborincana) of Hebdomadis serogroup. The agglutination titres of McAb Af2 and McAb Bb2 were 1:640-1:2,500,000 and 1:320-1:2,500,000, respectively. The two McAbs did not agglutinate with serovar kabura of Hebdomadis serogroup, they did not agglutinate with 11 serovars of Sejroe serogroup, 4 serovars of Mini serogroup, 18 representive serovars of L. interrogans in 18 serogroups, L. biflexa strain Patoc I and Leptonema illini. So it was found that McAb Af2 and McAb Bb2 showed partial serogroup specificity for Hebdomadis by agglutination.
七日热群是在中国发现的主要血清群之一。在四川，该血清群似乎与当地钩端螺旋体病的暴发密切相关。用七日热群七日热血清型245菌株的外膜对BALB/c小鼠进行脾内免疫。脾细胞与SP2/0骨髓瘤细胞融合，通过杂交瘤技术产生了两种单克隆抗体Af2和Bb2。免疫扩散法分别鉴定单克隆抗体Af2和单克隆抗体Bb2为IgG1和IgG3。通过显微镜凝集试验确定这两种单克隆抗体的特异性；二者均与七日热血清群的8个血清型（七日热、诺纳、坎巴莱、克雷马斯托斯、沃斯福尔迪、朱尔斯、马鲁博林卡纳）发生反应。单克隆抗体Af2和单克隆抗体Bb2的凝集效价分别为1:640 - 1:2,500,000和1:320 - 1:2,500,000。这两种单克隆抗体不与七日热血清群的卡布拉血清型发生凝集，不与 sejroe血清群的11个血清型、Mini血清群的4个血清型、问号状钩端螺旋体18个血清群中的18个代表性血清型、双曲钩端螺旋体Patoc I菌株和伊利诺伊细螺旋体发生凝集。因此，通过凝集发现单克隆抗体Af2和单克隆抗体Bb2对七日热血清群表现出部分血清群特异性。
