Adler B, Faine S
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1983 Sep;255(2-3):317-22.
Mouse antisera to Leptospira interrogans serovar pomona or L. biflexa serovar patoc agglutinated homologous leptospires but not leptospires of serovars from any heterologous serogroup tested. When tested by enzyme immunoassay (EIA), pomona and patoc antisera reacted with all serovars tested from L. interrogans, L. biflexa or L. illini species. A non-agglutinating hybridoma-derived monoclonal antibody reacted by EIA only with serovars of the pathogenic L. interrogans species but not with L. biflexa nor L. illini. The results demonstrated the presence of both genus and species specific non-agglutinating leptospiral antigens which could be detected only by EIA.
针对问号钩端螺旋体波摩那血清型或双曲钩端螺旋体帕托克血清型的小鼠抗血清能凝集同源钩端螺旋体，但不能凝集来自任何测试的异源血清群的血清型钩端螺旋体。当通过酶免疫测定（EIA）进行测试时，波摩那和帕托克抗血清与来自问号钩端螺旋体、双曲钩端螺旋体或伊利尼钩端螺旋体物种的所有测试血清型发生反应。一种非凝集性的杂交瘤衍生单克隆抗体仅通过EIA与致病性问号钩端螺旋体物种的血清型发生反应，而不与双曲钩端螺旋体或伊利尼钩端螺旋体发生反应。结果表明存在仅能通过EIA检测到的属特异性和种特异性非凝集性钩端螺旋体抗原。
