Adler B, Ballard S A, Miller S J, Faine S
Department of Microbiology, Monash University, Melbourne, Australia.
FEMS Microbiol Immunol. 1989 Mar;1(4):213-8. doi: 10.1111/j.1574-6968.1989.tb02385.x.
Monoclonal antibodies with two kinds of specificities, produced against Leptospira interrogans serovar pomona, were studied by agglutination and immunoblotting. Antibodies reacted either exclusively with serovar pomona or with all members of the Pomona serogroup, but none of the antibodies reacted with representative serovars of other serogroups. Both antibodies recognized epitopes on purified lipopolysaccharide (LPS) from serovar pomona. In immunoblotting experiments the serogroup specific antibody recognized both the major LPS bands of 21 kDa and 26 kDa whereas the serovar specific antibodies reacted only with the 26 kDa band, thus localizing serovar specificity in the 26 kDa band and serogroup specific epitopes on at least two different LPS subunits.
通过凝集试验和免疫印迹法研究了针对问号钩端螺旋体波摩那群血清型制备的具有两种特异性的单克隆抗体。这些抗体要么仅与波摩那群血清型发生反应，要么与波摩那群的所有成员发生反应，但没有一种抗体与其他血清群的代表性血清型发生反应。两种抗体均识别波摩那群血清型纯化脂多糖（LPS）上的表位。在免疫印迹实验中，血清群特异性抗体识别21 kDa和26 kDa的主要LPS条带，而血清型特异性抗体仅与26 kDa条带发生反应，从而将血清型特异性定位在26 kDa条带中，并且血清群特异性表位位于至少两个不同的LPS亚基上。
