以1,5-双（对羟基苯亚甲基）硫代碳酰肼为辣根过氧化物酶底物催化分光光度法测定茶饮料中的抗坏血酸

Catalytic spectrophotometric determination of ascorbic acid in tea drink with 1,5-bis(p-hydroxybenzaldene)thiocarbohydrazone as the substrate for horseradish peroxidase.

作者信息

Tang Bo, Wang Yan, Du Ming, Ge Jie-Chao, Chen Zhen-Zhen

机构信息

Department of Chemistry, Shandong Normal University, Jinan, 250014, People's Republic of China.

出版信息

J Agric Food Chem. 2003 Jul 16;51(15):4198-201. doi: 10.1021/jf030012e.

DOI:10.1021/jf030012e

PMID:12848484

Abstract

1,5-bis(p-Hydroxybenzaldene)thiocarbohydrazone (BHBTZ) had been synthesized and used as a new kind of substrate for horseradish peroxidase (HRP). The experiment results showed that ascorbic acid (AsA) had strongly inhibiting effect on the BHBTZ-H(2)O(2)-HRP reaction system, so AsA had been spectrophotomericly determined as the inhibitor by enzymatic inhibition method. Under experimental conditions, the linear relationship between DeltaA(386) and AsA concentration was in the range of 0.82-18.0 microg ml(-1) with a detection limit of 0.245 microg ml(-1). The effect of interferences on AsA determination was investigated. The proposed method was successfully applied to the determination of AsA in tea drink.

摘要

1,5-双（对羟基苯亚甲基）硫代碳腙（BHBTZ）已被合成并用作辣根过氧化物酶（HRP）的一种新型底物。实验结果表明，抗坏血酸（AsA）对BHBTZ-H₂O₂-HRP反应体系有强烈的抑制作用，因此采用酶抑制法通过分光光度法将AsA测定为抑制剂。在实验条件下，ΔA₃₈₆与AsA浓度之间的线性关系在0.82 - 18.0 μg ml⁻¹范围内，检测限为0.245 μg ml⁻¹。研究了干扰对AsA测定的影响。该方法成功应用于茶饮料中AsA的测定。