Prasain Jeevan K, Jones Kenneth, Kirk Marion, Wilson Landon, Smith-Johnson Michelle, Weaver Connie, Barnes Stephen
Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
J Agric Food Chem. 2003 Jul 16;51(15):4213-8. doi: 10.1021/jf030174a.
The kudzu vine (Pueraria sp.) is a rich source of isoflavones. Dietary supplements based on kudzu have become commercially available. In the present study, liquid chromatography coupled with negative and positive electrospray ionization tandem mass spectrometry (MS/MS) and diode array detection (DAD) has been used for the detection and characterization of isoflavonoids in kudzu dietary supplements (KDS). The MS/MS spectrum of the protonated ion of puerarin showed characteristic product ions of the C-glycoside unit itself, whereas daidzin generated an abundant Y(0)(+) aglycon ion in its product ion spectrum. A base peak due to the loss of 120 Da M + H - 120 is the diagnostic ion for C-glycosides. Neutral loss scans allowed for the detection of other C- and O-glycosides in the methanolic extract of KDS, and their structures have been proposed. The concentration of isoflavonoids in the methanolic extract of commercially available KDS was quantified by using DAD-HPLC. Puerarin, rather than daidzin, was the most abundant component (8.44-30.60 mg/capsule) in commercially available KDS.
葛根（野葛属）是异黄酮的丰富来源。以葛根为基础的膳食补充剂已上市销售。在本研究中，液相色谱结合正负电喷雾电离串联质谱（MS/MS）和二极管阵列检测（DAD）已用于葛根膳食补充剂（KDS）中异黄酮类化合物的检测和表征。葛根素质子化离子的MS/MS谱显示了C-糖苷单元本身的特征性产物离子，而大豆苷在其产物离子谱中产生了丰富的Y(0)(+)苷元离子。由于失去120 Da M + H - 120产生的基峰是C-糖苷的诊断离子。中性丢失扫描可检测KDS甲醇提取物中的其他C-和O-糖苷，并已推测出它们的结构。通过DAD-HPLC对市售KDS甲醇提取物中异黄酮类化合物的浓度进行了定量。葛根素而非大豆苷是市售KDS中含量最丰富的成分（8.44 - 30.60 mg/胶囊）。
