Naigowit P, Maneeboonyoung W, Wongroonsub P, Chaowagul V, Kanai K
National Institute of Health, Department of Medical Sciences, Bamrasnaradura Hospital, Nonthaburi, Thailand.
Jpn J Med Sci Biol. 1992 Oct-Dec;45(5-6):215-30. doi: 10.7883/yoken1952.45.215.
A nation-wide survey was conducted to see the prevalence of serosensitivity to Pseudomonas pseudomallei antigens by indirect hemagglutination (IHA) and indirect immunofluorescent assay (IFA) for IgG and IgM. Serum samples were collected from blood donors in eight selected areas and bacteriologically confirmed melioidosis patients in Ubon Ratchathani province. The distribution patterns of antibody titers were compared among the survey areas with cut-off points set at 1:160 for IHA, 1:4 for IFA-IgM and 1:32 for IFA-IgG. These cut-off points were decided by ROC (Receiver Operating Characteristics) analysis. The specificity (% true negative reactions) of each serological test in the general population differed significantly among survey areas, possibly reflecting the extent of inapparent infection in each community. IFA was more successful than IHA in differentiating between negative from positive reactions. The survey classified the areas into endemic (Khon Kaen, Ubon Ratchathani), transported (Bangkok), and non-endemic (other provinces) types.
开展了一项全国性调查，通过间接血凝试验（IHA）以及针对IgG和IgM的间接免疫荧光试验（IFA）来了解对类鼻疽杆菌抗原的血清敏感性流行情况。从八个选定地区的献血者以及乌汶叻差他尼府经细菌学确诊的类鼻疽病患者中采集血清样本。比较了各调查地区抗体滴度的分布模式，IHA的截断点设定为1:160，IFA-IgM的截断点设定为1:4，IFA-IgG的截断点设定为1:32。这些截断点是通过ROC（受试者工作特征）分析确定的。各血清学检测在普通人群中的特异性（真阴性反应百分比）在各调查地区存在显著差异，这可能反映了每个社区隐性感染的程度。在区分阴性和阳性反应方面，IFA比IHA更成功。该调查将各地区分为地方性流行区（孔敬、乌汶叻差他尼）、传入区（曼谷）和非地方性流行区（其他省份）。
