内毒素抗原在酶联免疫吸附测定中用于诊断类鼻疽杆菌感染（类鼻疽病）。
Use of endotoxin antigens in enzyme-linked immunosorbent assay for the diagnosis of P. pseudomallei infections (melioidosis).
作者信息
Petkanjanapong V, Naigowit P, Kondo E, Kanai K
机构信息
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
出版信息
Asian Pac J Allergy Immunol. 1992 Dec;10(2):145-50.
An enzyme-linked immunosorbent assay (ELISA) with endotoxin preparations of P. pseudomallei as antigen was developed for detection of IgG antibodies specific to melioidosis. Forty-seven sera of bacteriologically confirmed melioidosis patients, 55 non-melioidosis sera and 50 sera of healthy blood donors from non-endemic areas were subjected to this assay in comparison with indirect hemagglutination assay (IHA). The data were treated by receiver operating characteristics analysis. The sensitivity, specificity and accuracy in this ELISA were 95.7%, 94.2%, and 94.7%, respectively, with cut-off value of OD = 0.312 at 490 nm. Meanwhile, those in IHA were 81.0%, 91.4%, and 88.1%, respectively, with a cut-off value of > or = 1:160. From these results, the ELISA was judged to be more reliable than IHA as the seroassay for diagnosis of melioidosis.
开发了一种以类鼻疽杆菌内毒素制剂为抗原的酶联免疫吸附测定（ELISA），用于检测类鼻疽病特异性IgG抗体。将47份经细菌学确诊的类鼻疽病患者血清、55份非类鼻疽病血清和50份来自非流行地区的健康献血者血清进行该测定，并与间接血凝试验（IHA）进行比较。数据采用受试者工作特征分析进行处理。该ELISA的敏感性、特异性和准确性分别为95.7%、94.2%和94.7%，490nm处的临界值为OD = 0.312。同时，IHA的敏感性、特异性和准确性分别为81.0%、91.4%和88.1%，临界值为≥1:160。根据这些结果，ELISA被判定为比IHA更可靠的类鼻疽病血清学诊断方法。
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