Fang Li-qun, Zhang Pan-he, Yang Bao-an, Wu Xiao-ming, Zhao Qiu-min, Liu Wei, Liu Hong, Deng Yong-qiang, Zhan Lin, Han Wei-guo, Lu Fu-shuang, Wu Jin-song, Yang Hong, Zhu Qing-yu, Cao Wu-chun
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2003 Jun;24(6):484-6.
To explore the temporal profile of serum antibody against coronavirus in patients with severe acute respiratory syndrome (SARS), and to evaluate the reliability of indirect immuno-fluorescence assay (IFA) in the diagnosis of SARS.
Clinically confirmed SARS patients, suspected SARS patients, and controls were included in the study. IFA was used to detect the serum antibody against SARS coronavirus. General information about the subjects was collected using a standard questionnaire.
The positive rates of specific IgG and IgM against SARS virus within 10 days after onset of the disease were 55.1% and 16.3% respectively and then increased up to 89.8% for IgG and 65.3% for IgM. After 25 days of the onset of the disease, 90.9% patients became positive for both IgG and IgM. Results from chi-square for trend test revealed that the positive rates of both IgG and IgM increased with time (chi(2) for trend = 16.376, P = 0.00005 for IgG; chi(2) for trend = 28.736, P = 0.00000 for IgM). Sensitivity, specificity and agreement value of IFA regarding the diagnosis of SARS were all higher than 90%.
IFA can be used to assist diagnosis of SARS after 10 days of the onset of disease.
探讨严重急性呼吸综合征（SARS）患者血清中抗冠状病毒抗体的时间变化规律，并评估间接免疫荧光法（IFA）在SARS诊断中的可靠性。
研究纳入临床确诊的SARS患者、疑似SARS患者及对照组。采用IFA检测血清中抗SARS冠状病毒抗体。通过标准问卷收集受试者的一般信息。
发病后10天内，抗SARS病毒特异性IgG和IgM的阳性率分别为55.1%和16.3%，之后IgG阳性率升至89.8%，IgM阳性率升至65.3%。发病25天后，90.9%的患者IgG和IgM均呈阳性。趋势检验的卡方结果显示，IgG和IgM的阳性率均随时间增加（IgG的趋势卡方值=16.376，P=0.00005；IgM的趋势卡方值=28.736，P=0.00000）。IFA诊断SARS的敏感性、特异性和一致性值均高于90%。
发病10天后，IFA可用于辅助SARS的诊断。
