Alves V A, Wakamatsu A, Kanamura C T, Magalhäes E S, Siqueira S A
Laboratório de Imunohistoquímica, Instituto Adolfo Lutz, São Paulo.
Rev Hosp Clin Fac Med Sao Paulo. 1992 Jan-Feb;47(1):19-24.
The fixation in ethanol or formalin for trypsin digestion in immunohistochemical detection of cytokeratins and vimentin was assessed in a case of ovarian cystadenofibrocarcinoma. Superior reactivity for both markers was achieved in ethanol-fixed sections, even in samples stocked up to 60 days. Cytokeratin reaction in formalin-fixed sections was better when trypsin was used. However, this digestion was deleterious to vimentin detection. These data are presented to alert surgeons and oncologists on the relevance of fixation of specimens suspicious for neoplasia, since different epitopes may require different fixatives and the inadequate choice in the operative room may impart difficulties when immunohistochemistry is necessary.
在一例卵巢囊腺纤维癌中，评估了在细胞角蛋白和波形蛋白免疫组化检测中用于胰蛋白酶消化的乙醇或福尔马林固定情况。在乙醇固定的切片中，两种标志物均表现出更好的反应性，即使是保存长达60天的样本。当使用胰蛋白酶时，福尔马林固定切片中的细胞角蛋白反应更好。然而，这种消化对波形蛋白检测有害。呈现这些数据是为了提醒外科医生和肿瘤学家注意对可疑肿瘤标本进行固定的相关性，因为不同的表位可能需要不同的固定剂，而在手术室选择不当可能会在需要进行免疫组化时带来困难。
