Boon M E, Kok L P
Leiden Cytology and Pathology Laboratory, Leiden, The Netherlands.
Eur J Morphol. 1991;29(3):173-80.
Formalin is hazardous for the environment and for the laboratory personnel and deleterious to cytoskeleton proteins. The pathology and anatomy laboratory can be formalin-free when Kryofix is used as a substitute fixative. In four years experience with Kryofix, we learned that immunostaining on paraffin sections is reproducible and of excellent quality. The current study concerns the comparison of interfilament staining on paraffin sections made from formalin or from Kryofix-treated tumors. For both keratin and vimentin there were false-negative findings in the formalin-fixed tumors. Of these two, vimentin was het most susceptible to formalin fixation. Three of the ten adenocarcinomas were positive for both keratin and vimentin, indicating that this double staining pattern is not uncommon. Postfixation of Kryofix-treated tissue with formalin results in false-negative immunostaining.
福尔马林对环境和实验室人员有害，并且会损害细胞骨架蛋白。当使用Kryofix作为替代固定剂时，病理和解剖实验室可以不使用福尔马林。在使用Kryofix的四年经验中，我们了解到石蜡切片上的免疫染色具有可重复性且质量优异。当前的研究涉及对由福尔马林或Kryofix处理的肿瘤制成的石蜡切片上的丝间染色进行比较。对于角蛋白和波形蛋白，在福尔马林固定的肿瘤中均出现了假阴性结果。在这两者中，波形蛋白对福尔马林固定最为敏感。十例腺癌中有三例角蛋白和波形蛋白均呈阳性，表明这种双重染色模式并不罕见。用福尔马林对Kryofix处理过的组织进行后固定会导致免疫染色假阴性。
