Yamaoka G, Taoka T, Arai T, Kiuchi H, Kawanishi K
Department of Clinical Laboratory, Kagawa Medical School.
Rinsho Byori. 1992 Nov;40(11):1224-6.
We examined alkaline phosphatase (ALP) activity in the HL-60 cell induced by retinoic acid (RA) and recombinant human granulocyte colony-stimulating factor (rhG-CSF). rhG-CSF induced a small but significant increase of NBT-reducing ability and ALP activity of the HL-60 cells. Among various inducers of cell differentiation, 1,25(OH)2D3 and dimethylsulfoxide (DMSO) caused the increase of the NBT-reducing ability and the suppression of ALP activity induced by rhG-CSF, while RA enhanced both of them. Protein kinase C inhibitors (H-7 and staurosporine) but not a protein kinase A inhibitor (HA1004) significantly suppressed the ALP activity induced by the simultaneous treatment with RA and rhG-CSF.
我们检测了维甲酸（RA）和重组人粒细胞集落刺激因子（rhG-CSF）诱导的HL-60细胞中的碱性磷酸酶（ALP）活性。rhG-CSF诱导HL-60细胞的NBT还原能力和ALP活性出现小幅但显著的增加。在各种细胞分化诱导剂中，1,25(OH)2D3和二甲基亚砜（DMSO）导致NBT还原能力增加，并抑制rhG-CSF诱导的ALP活性，而RA则增强了这两者。蛋白激酶C抑制剂（H-7和星形孢菌素）而非蛋白激酶A抑制剂（HA1004）显著抑制了RA和rhG-CSF联合处理诱导的ALP活性。
