Taoka T, Yamaoka G, Arai T, Kiuchi H, Tanaka T, Takahara J, Kawanishi K, Irino S
Department of Clinical Laboratory, Kagawa Medical School, Japan.
Leuk Res. 1993 Aug;17(8):695-8. doi: 10.1016/0145-2126(93)90076-w.
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) not only enhanced the growth of HL-60 cells, but also significantly increased NBT-reducing ability and alkaline phosphatase (ALP) activity of the cells, which were enhanced by the treatment with retinoic acid (RA). Protein kinase C inhibitors (H-7 and staurosporine) significantly suppressed this induction of ALP. The pretreatment with RA followed by rhG-CSF treatment showed almost the same degree of ALP activity as that induced by the simultaneous treatment with RA and rhG-CSF. This study suggests that RA and rhG-CSF are the potent inducers of ALP activity of HL-60 cells and protein kinase C is supposed to have a role in this induction of ALP.
重组人粒细胞集落刺激因子（rhG-CSF）不仅能促进HL-60细胞的生长，还能显著提高细胞的硝基蓝四唑（NBT）还原能力和碱性磷酸酶（ALP）活性，而视黄酸（RA）处理可增强这些能力。蛋白激酶C抑制剂（H-7和星形孢菌素）显著抑制了这种ALP的诱导。先用RA预处理，再用rhG-CSF处理，其ALP活性与RA和rhG-CSF同时处理诱导的活性几乎相同。本研究表明，RA和rhG-CSF是HL-60细胞ALP活性的有效诱导剂，蛋白激酶C可能在这种ALP诱导中起作用。
