Basu Pranab S, Majhi Ramdhan, Batabyal Sandip K
Indian Institute of Chemical Biology 4, Raja S.C. Mullick Road, Jadavpur, 700032 Calcutta, India.
Clin Biochem. 2003 Jul;36(5):373-6. doi: 10.1016/s0009-9120(03)00050-x.
The present investigation was designed to distinguish prostate cancer and benign prostate hyperplasia by lectin-prostate specific antigen (PSA) binding.
The quantitative precipitin method of concanavalin A (Con A)-carbohydrate interaction was explored with the serum PSA of patients suffering from prostatic complications.
The carbohydrate content in the precipitate after binding of Con A with serum PSA of prostate cancer was significantly lower than that of benign prostate hyperplasia. This may be due to altered sugar chain structure or less glycosylation of PSA in prostate cancer.
We conclude that a serum value <3.0 microg/ml of the carbohydrate content of Con A-PSA precipitate indicates strong suspicion for prostate cancer and this cut off level is effective in reducing the rate of unnecessary biopsies in men with total PSA value between 4.0 to 10.0 ng/ml.
本研究旨在通过凝集素 - 前列腺特异性抗原（PSA）结合来区分前列腺癌和良性前列腺增生。
采用刀豆球蛋白A（Con A）与碳水化合物相互作用的定量沉淀法，对患有前列腺疾病的患者血清PSA进行研究。
Con A与前列腺癌患者血清PSA结合后沉淀物中的碳水化合物含量明显低于良性前列腺增生患者。这可能是由于前列腺癌中PSA糖链结构改变或糖基化程度降低所致。
我们得出结论，Con A - PSA沉淀物中碳水化合物含量血清值<3.0微克/毫升强烈怀疑为前列腺癌，该临界值可有效降低总PSA值在4.0至10.0纳克/毫升男性患者的不必要活检率。
