Kolho E, Naukkarinen R, Krusius T
Finnish Red Cross Blood Transfusion Service, Helsinki.
Transfus Med. 1992 Sep;2(3):243-8. doi: 10.1111/j.1365-3148.1992.tb00163.x.
A retrospective study was carried out on the recipients of 73 units of blood from 53 donors found reactive for anti-HCV. The donors were screened with anti-HCV enzyme-linked immunosorbent assay (ELISA C-100) and reactivity was confirmed with the first generation recombinant immunoblot assay (RIBA I). Fifty-two patients were recipients of blood from donors reacting as RIBA I 'indeterminate' and 21 of blood from RIBA I 'positive' donors. Only three recipients (5.8%) from 'indeterminate' donors were anti-HCV positive indicating that such donors are very seldom infectious. Eleven (52.4%) recipients from 'positive' donors had antibodies to HCV, indicating that not all RIBA-positive donors are necessarily infectious. Pretransfusion samples of the seropositive recipients were unavailable. All samples were analyzed with the first generation ELISA and with either the second-generation ELISA or RIBA (RIBA II) in order to evaluate test sensitivity. RIBA II was more sensitive than RIB I. One RIBA I indeterminate donor was positive by RIBA II. His recipient had antibodies to HCV. Twelve RIBA I indeterminate and three RIBA I positive donors were negative by RIBA II. All their recipients were anti-HCV negative. The second-generation ELISA was also shown to be more sensitive than ELISA C-100. The second-generation ELISA detected six confirmed anti-HCV positive recipients who were negative by ELISA C-100.
对53名抗丙型肝炎病毒（anti-HCV）检测呈反应性的献血者所提供的73单位血液的受血者进行了一项回顾性研究。献血者通过抗-HCV酶联免疫吸附测定（ELISA C-100）进行筛查，并用第一代重组免疫印迹测定（RIBA I）确认反应性。52名患者接受了来自RIBA I检测结果为“不确定”的献血者的血液，21名患者接受了来自RIBA I检测结果为“阳性”的献血者的血液。来自“不确定”献血者的受血者中只有3人（5.8%）抗-HCV呈阳性，这表明这类献血者很少具有传染性。来自“阳性”献血者的受血者中有11人（52.4%）具有抗HCV抗体，这表明并非所有RIBA阳性献血者都一定具有传染性。血清学阳性受血者的输血前样本无法获取。所有样本均采用第一代ELISA以及第二代ELISA或RIBA（RIBA II）进行分析，以评估检测灵敏度。RIBA II比RIBA I更灵敏。一名RIBA I检测结果为不确定的献血者经RIBA II检测呈阳性。其受血者具有抗HCV抗体。12名RIBA I检测结果为不确定的献血者和3名RIBA I检测结果为阳性的献血者经RIBA II检测呈阴性。他们所有的受血者抗-HCV均为阴性。第二代ELISA也比ELISA C-100更灵敏。第二代ELISA检测出6名经确认抗-HCV呈阳性但ELISA C-100检测为阴性的受血者。
