Mirelman D, Keren Z, Bracha R
Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Arch Med Res. 1992;23(2):49-53.
A novel and unique mAb (318-28) which specifically interacts with a 60 kDa antigen that is found only on the surfaces of non-pathogenic (NP) strains of E. histolytica has been recently characterized. The antigen appears to be present also on (NP) cyst forms of amebas but was not detected on any of the various (P)-strains tested. It was also not found on other Entamoeba species such as Moshkovskii, Laredo, Huff, Coli, Gingivalis, or Invadens. Clinical trails for the differentiation of (P) and (NP) amebas directly from stools using this mAb are in progress. Cloning of the gene encoding for the (NP)-specific antigen was achieved after screening with mAb 318-28 a lambda gt11 expression library of NP strain SAW 1734R. No sequence homology to any known protein was found in data bases.
最近鉴定出一种新型独特的单克隆抗体（318-28），它能特异性地与一种60 kDa抗原相互作用，该抗原仅在溶组织内阿米巴非致病性（NP）菌株的表面发现。这种抗原似乎也存在于阿米巴的（NP）包囊形式中，但在任何测试的各种（P）菌株中均未检测到。在其他内阿米巴物种如莫氏内阿米巴、拉雷多内阿米巴、赫夫内阿米巴、结肠内阿米巴、牙龈内阿米巴或侵袭内阿米巴中也未发现。目前正在进行使用这种单克隆抗体直接从粪便中区分（P）和（NP）阿米巴的临床试验。在用单克隆抗体318-28筛选NP菌株SAW 1734R的λgt11表达文库后，实现了编码（NP）特异性抗原的基因克隆。在数据库中未发现与任何已知蛋白质的序列同源性。
