Berni Jimena, Rabossi Alejandro, Quesada-Allué Luis A
Instituto de Investigaciones Bioquímicas-Fundación Campomar, CONICET and FCEyN University of Buenos Aires, Patricias Argentinas 435 (1405), Buenos Aires, Argentina.
J Econ Entomol. 2003 Jun;96(3):662-8. doi: 10.1093/jee/96.3.662.
A laboratory bioassay was developed to determine both the chemical toxicity and the phototoxicity of the xanthene dye, phloxine B (D&C Red No 28), to the immature stages of the Mediterranean fruit fly, Certitis capitata (Wiedemann). An additional goal was to find out which main tissues are affected first. A low, but significant, level of toxicity was observed when the insects were maintained in the dark: at the point of adult ecdysis, the LC50 was 11.03 mM. As expected, after 8-h exposure of late larva III to light, a high level of mortality was produced (LC50 at ecdysis: 0.45 mM) as a dose-dependent function of dye concentration. At sublethal concentrations of the dye, the surviving insects showed a number of physiological abnormalities. Phloxine B appeared to mainly affect the larval longitudinal muscles as well as the abdominal muscles of ecdysing adults, giving rise to abnormal puparia and failed adult ecdysis, respectively. Moreover, a significant phloxine B-dependent delay in the jumping of surviving larvae for dispersal was documented. This could be attributed to a delay in attaining a threshold weight for jumping and/or to abnormalities in neuromuscular coordination, thus reinforcing the idea of pleiotropic effects of the dye.
已开发出一种实验室生物测定法，以确定呫吨染料荧光素B（D&C红28号）对地中海实蝇（Ceratitis capitata (Wiedemann)）未成熟阶段的化学毒性和光毒性。另一个目标是找出哪些主要组织首先受到影响。当昆虫在黑暗中饲养时，观察到低但显著的毒性水平：在成虫蜕皮时，LC50为11.03 mM。正如预期的那样，在三龄后期幼虫暴露于光照8小时后，产生了高死亡率（蜕皮时的LC50：0.45 mM），这是染料浓度的剂量依赖性函数。在染料的亚致死浓度下，存活的昆虫表现出一些生理异常。荧光素B似乎主要影响幼虫的纵向肌肉以及蜕皮成虫的腹部肌肉，分别导致异常蛹化和成虫蜕皮失败。此外，记录到存活幼虫跳跃以进行扩散的过程中，荧光素B导致了显著的延迟。这可能归因于达到跳跃阈值体重的延迟和/或神经肌肉协调异常，从而强化了染料具有多效性作用的观点。
