IIBBA-CONICET, Química Biológica-FCEyN-Universidad de Buenos Aires and Fundación Instituto Leloir, Buenos Aires, Argentina.
Photochem Photobiol. 2010 May-Jun;86(3):639-44. doi: 10.1111/j.1751-1097.2009.00704.x. Epub 2010 Feb 23.
Immature stages of Ceratitis capitata were tested as a model for hematoporphyrin IX (HP IX) phototoxicity. The lethal concentration 50 (LC(50)) of HP IX in the food was determined during postembryonic development until adult emergence as 0.173 mm (95% CI: 0.138-0.209). The corresponding HP IX LC(50) during the dispersal period alone was 0.536 mm (95% CI: 0.450-0.633). HP IX toxicity was compared against Phloxine B (PhB) (0.5 mm). HP IX elicited a mortality of 90.87%, which was mainly concentrated during prepupal and early pupal stages. PhB mortality was much lower (56.88%) and occurred mainly during the adult pharate stage. A direct correlation between light-dependent HP IX mortality, evidence of reactive oxygen species (ROS) and lipid peroxidation (conjugated dienes and thiobarbituric acid reactive substances) was established in C. capitata larvae. ROS were found to be very significant in both the brain and in the gut.
地中海实蝇的未成熟阶段被用作血卟啉 IX（HP IX）光毒性的模型。在胚胎后发育直至成虫出现期间，食物中 HP IX 的致死浓度 50（LC50）确定为 0.173 毫米（95%CI：0.138-0.209）。单独在扩散期的相应 HP IX LC50 为 0.536 毫米（95%CI：0.450-0.633）。将 HP IX 毒性与 Phloxine B（PhB）（0.5 毫米）进行了比较。HP IX 引起的死亡率为 90.87%，主要集中在预蛹和早期蛹期。PhB 的死亡率要低得多（56.88%），主要发生在成虫的预蛹阶段。在 C. capitata 幼虫中，光依赖性 HP IX 死亡率、活性氧（ROS）和脂质过氧化（共轭二烯和硫代巴比妥酸反应物质）的证据之间建立了直接相关性。在大脑和肠道中均发现 ROS 非常显著。
