Sebald Eiman, Krueger Richard, King Lily M, Cohn Daniel H, Krakow Deborah
Department of Obstetrics and Gynecology, Burns and Allen Cedars-Sinai Research Institute, 8700 Beverly Boulevard, Los Angeles, CA 90048, USA.
Gene. 2003 Jun 5;311:147-51. doi: 10.1016/s0378-1119(03)00584-5.
ADP-ribosylation factors (ARFs) and ARF-like proteins (ARLs) are part of the ARF family within the RAS superfamily of regulatory GTPases. Guanine nucleotide binding proteins or GTPases are involved in a diverse spectrum of cellular activities, including regulating cell growth and signal transduction, organization of the cytoskeleton and regulating membrane trafficking along the exocytic and endocytic pathways. ARL proteins share 40-60% sequence identity with the ARF proteins, but ARLs can be distinguished from ARFs based on expression patterns and biological functions. We have identified a new ARL, ARL8, from a fetal cartilage cDNA library. ARL8 contains six exons and five introns, and encodes a 179 amino acid protein that shares homology to the other ARL proteins, especially ARL5. It also shows significant homology with orthologous proteins found in Mus musculus and Drosophila melanogaster. The expression pattern of the mouse ortholog revealed differential tissue expression and an alternate transcript was seen in brain that was age-dependent. ARL8 is an additional member of a family of closely related proteins that are conserved both within the family and across species.
ADP核糖基化因子（ARFs）和ARF样蛋白（ARLs）是RAS超家族中调节性GTP酶的ARF家族的一部分。鸟嘌呤核苷酸结合蛋白或GTP酶参与多种细胞活动，包括调节细胞生长和信号转导、细胞骨架的组织以及调节沿胞吐和胞吞途径的膜运输。ARL蛋白与ARF蛋白具有40%-60%的序列同一性，但基于表达模式和生物学功能，ARLs可与ARFs区分开来。我们从胎儿软骨cDNA文库中鉴定出一种新的ARL，即ARL8。ARL8包含六个外显子和五个内含子，编码一种179个氨基酸的蛋白质，该蛋白质与其他ARL蛋白具有同源性，尤其是ARL5。它还与小家鼠和黑腹果蝇中发现的直系同源蛋白具有显著同源性。小鼠直系同源物的表达模式显示出不同的组织表达，并且在大脑中发现了一种与年龄相关的可变转录本。ARL8是一个密切相关蛋白家族的另一个成员，该家族在家族内部和跨物种中都是保守的。
