Soruri Afsaneh, Kim Soyoung, Kiafard Ziba, Zwirner Jörg
Department of Immunology, Georg-August-University Göttingen, Kreuzbergring 57, D-37075 Gottingen, Germany.
Immunol Lett. 2003 Jul 3;88(1):47-52. doi: 10.1016/s0165-2478(03)00052-x.
The anaphylatoxin C5a is a potent proinflammatory stimulus with immunomodulatory activities. Expression of its receptor C5aR (CD88) has been detected on cells of myeloid origin such as granulocytes and monocytes/macrophages. However, controversial results exist on the expression of C5aR on T and B lymphocytes as well as on mature dendritic cells (DC). The aim of the present study was to characterize expression of C5aR protein on myeloid and lymphoid cells in the mouse. For this purpose, rat monoclonal antibodies with specificity against the murine C5aR were generated. Using these reagents a distinct amount of C5aR antigen was observed on neutrophils and macrophages. In contrast, C5aR protein was not detectable on resting or stimulated murine T or B lymphocytes. Furthermore, no C5aR protein could be observed on splenic CD11c positive DC which have been classified in the literature as relatively mature. Taken together, our results suggest that in the mouse expression of C5aR protein may be restricted to leukocytes of myeloid origin whereas previous evidence for C5aR expression on lymphoid cells may be reevaluated.
过敏毒素C5a是一种具有免疫调节活性的强效促炎刺激物。在粒细胞和单核细胞/巨噬细胞等髓系来源的细胞上已检测到其受体C5aR（CD88）的表达。然而，关于C5aR在T和B淋巴细胞以及成熟树突状细胞（DC）上的表达存在有争议的结果。本研究的目的是表征小鼠髓系和淋巴细胞上C5aR蛋白的表达。为此，制备了针对小鼠C5aR具有特异性的大鼠单克隆抗体。使用这些试剂，在中性粒细胞和巨噬细胞上观察到了不同量的C5aR抗原。相比之下，在静息或受刺激的小鼠T或B淋巴细胞上未检测到C5aR蛋白。此外，在脾脏CD11c阳性DC上也未观察到C5aR蛋白，这些DC在文献中被归类为相对成熟的细胞。综上所述，我们的结果表明，在小鼠中，C5aR蛋白的表达可能仅限于髓系来源的白细胞，而先前关于C5aR在淋巴细胞上表达的证据可能需要重新评估。
