用于特异性检测雷斯顿埃博拉病毒核蛋白的抗原捕获酶联免疫吸附测定。
Antigen capture enzyme-linked immunosorbent assay for specific detection of Reston Ebola virus nucleoprotein.
作者信息
Ikegami Tetsuro, Niikura Masahiro, Saijo Masayuki, Miranda Mary E, Calaor Alan B, Hernandez Marvin, Acosta Luz P, Manalo Daria L, Kurane Ichiro, Yoshikawa Yasuhiro, Morikawa Shigeru
机构信息
Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan.
出版信息
Clin Diagn Lab Immunol. 2003 Jul;10(4):552-7. doi: 10.1128/cdli.10.4.552-557.2003.
Abstract
Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.
摘要
抗原捕获酶联免疫吸附测定（ELISA）是快速检测埃博拉病毒最有用的方法之一。我们之前利用单克隆抗体（MAb）3-3D开发了一种抗原捕获ELISA，该抗体不仅能与扎伊尔埃博拉病毒（EBO-Z）的核蛋白（NP）发生反应，还能与苏丹埃博拉病毒（EBO-S）和雷斯顿埃博拉病毒（EBO-R）的NP发生反应。在本研究中，我们利用两种针对EBO-R NP的新型MAb，即Res2-6C8和Res2-1D8，开发了抗原捕获ELISA。Res2-6C8和Res2-1D8分别识别EBO-R NP C端区域附近由4个和8个氨基酸残基组成的表位。使用这两种MAb的抗原捕获ELISA检测了EBO-R感染食蟹猴组织中的EBO-R NP。使用Res2-6C8和Res2-1D8的抗原捕获ELISA可用于快速检测EBO-R感染食蟹猴中的NP。
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Exp Anim. 2002 Oct;51(5):447-55. doi: 10.1538/expanim.51.447.
3
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