Molnár J, Fischer J, Nakamura M J
Institute of Microbiology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Antonie Van Leeuwenhoek. 1992 Nov;62(4):309-14. doi: 10.1007/BF00572599.
Chlorpromazine forms charge-transfer complexes with xanthene dyes in bacteria. These complexes permit the differentiation of Gram-positive and Gram-negative bacteria in both light and polarization microscopy. The birefringence induced by the charge-transfer complex might explain the molecular basis of bacterial staining. The charge-transfer complexes formed between chlorpromazine and xanthene dyes accumulate in the bacterial cell, mainly inside the bacterial cell wall. The complexes give the cells a color, which depends on the chemical composition of the staining structure, and in particular the polysaccharides of the cell wall in bacteria. Metachromatic granules were seen inside Gram-positive bacteria after chlorpromazine and rose bengal staining. Although the nature of these granules remains unclear, this type of binding may have a role in the inhibition of biochemical processes in the bacterial cells.
氯丙嗪在细菌中与呫吨染料形成电荷转移复合物。这些复合物使得在光学显微镜和偏振显微镜下均可区分革兰氏阳性菌和革兰氏阴性菌。电荷转移复合物诱导产生的双折射可能解释了细菌染色的分子基础。氯丙嗪与呫吨染料之间形成的电荷转移复合物积聚在细菌细胞内，主要是在细菌细胞壁内部。这些复合物赋予细胞一种颜色，该颜色取决于染色结构的化学成分，尤其是细菌细胞壁中的多糖。氯丙嗪和孟加拉玫瑰红染色后，在革兰氏阳性菌内部可见异染颗粒。尽管这些颗粒的性质尚不清楚，但这种结合类型可能在抑制细菌细胞中的生化过程中发挥作用。
