Fischer J, Emödy L
Acta Microbiol Acad Sci Hung. 1976;23(1):97-108.
Selective topo-optical staining of vicinal OH groups with aldehyde-bisulphite-toluidine blue (ABT) has been used for studying the molecular structural order of polysaccharide components in microbial cell walls and capsules. (i) By intensive metachromatic staining in the light microscope, the method demonstrates carbohydrates, containing free vicinal OH or acylaminohydroxyl groups. (ii) The birefringence induced by oriented toluidine blue binding of the ABT reaction, provides information about the linear order of the reacting carbohydrate chains, a possibility not offered by other ultrastructural methods. Furthermore the different optical character of birefringence induced by ABT is indicative of the presence of (a) tangentially oriented polysaccharide chains in the cell wall of the molds and bacterial capsules; or (b) radially oriented OH groups suggesting helical glycan chains of peptidoglucomannane and mucopeptide in the yeasts and bacterial cell walls.
醛亚硫酸氢盐 - 甲苯胺蓝（ABT）对相邻羟基进行选择性拓扑光学染色已被用于研究微生物细胞壁和荚膜中多糖成分的分子结构顺序。（i）通过在光学显微镜下进行强烈的异染性染色，该方法可显示含有游离相邻羟基或酰氨基羟基的碳水化合物。（ii）ABT反应中甲苯胺蓝定向结合所诱导的双折射，提供了有关反应性碳水化合物链线性顺序的信息，这是其他超微结构方法所不具备的可能性。此外，ABT诱导的双折射的不同光学特性表明：（a）霉菌细胞壁和细菌荚膜中存在切向排列的多糖链；或（b）径向排列的羟基表明酵母和细菌细胞壁中存在肽葡糖甘露聚糖和粘肽的螺旋聚糖链。
