Hessle Christina C, Andersson Bengt, Wold Agnes E
Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, SE-413 46 Göteborg, Sweden.
Cytokine. 2005 Jun 21;30(6):311-8. doi: 10.1016/j.cyto.2004.05.008. Epub 2005 Apr 7.
Pro-inflammatory cytokines secreted by tissue macrophages recruit polymorphonuclear leukocytes and evoke fever, cachexia and production of acute phase proteins. This study investigates whether Gram-positive and Gram-negative bacteria equally and efficiently trigger production of the pro-inflammatory cytokines IL-1 beta, IL-6, IL-8 and TNF-alpha in human monocytes. A range of aerobic and anaerobic Gram-positive and Gram-negative bacteria were killed by UV-light and added in different concentrations to human monocytes. Cytokines were measured in 24 h supernatants by ELISA. Gram-positive and Gram-negative bacteria were equally efficient inducers of IL-1 beta, but Gram-positive bacteria generated twice as much TNF-alpha as did Gram-negative bacteria (p<0.001 for 25 and 250 bacteria/cell). In contrast, Gram-negative bacteria induced at least twice as much IL-6 and IL-8 as did Gram-positive bacteria (p<0.001 for 2.5, 25 and 250 bacteria/cell). While the cytokine responses to LPS were similar to those induced by the corresponding amount of Gram-negative bacteria, the strong IL-1 beta and TNF-alpha responses to Gram-positive bacteria could not be induced by soluble peptidoglycan or lipotheicoic acid. The particular nature of the bacteria, thus seem to modify the response to Gram-positive bacterial components. The different cytokine profiles evoked by Gram-positive and Gram-negative bacteria might optimize clearance of bacteria that differ in cell wall structure.
组织巨噬细胞分泌的促炎细胞因子可募集多形核白细胞，并引发发热、恶病质和急性期蛋白的产生。本研究调查革兰氏阳性菌和革兰氏阴性菌是否能同等有效地触发人单核细胞中促炎细胞因子白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和肿瘤坏死因子-α（TNF-α）的产生。一系列需氧和厌氧的革兰氏阳性菌和革兰氏阴性菌经紫外线照射灭活后，以不同浓度添加到人单核细胞中。通过酶联免疫吸附测定法（ELISA）检测24小时培养上清液中的细胞因子。革兰氏阳性菌和革兰氏阴性菌诱导IL-1β产生的效率相同，但革兰氏阳性菌产生的TNF-α是革兰氏阴性菌的两倍（每细胞25和250个细菌时，p<0.001）。相比之下，革兰氏阴性菌诱导产生的IL-6和IL-8至少是革兰氏阳性菌的两倍（每细胞2.5、25和250个细菌时，p<0.001）。虽然细胞因子对脂多糖（LPS）的反应与相应数量的革兰氏阴性菌诱导的反应相似，但可溶性肽聚糖或脂磷壁酸无法诱导出对革兰氏阳性菌的强烈IL-1β和TNF-α反应。因此，细菌的特殊性质似乎会改变对革兰氏阳性菌成分的反应。革兰氏阳性菌和革兰氏阴性菌引发的不同细胞因子谱可能会优化对细胞壁结构不同的细菌的清除。
