Florek Ewa, Piekoszewski Wojciech, Wrzosek Jagna
Department of Toxicology, Karol Marcinkowski University of Medical Sciences, Dojzad 30, PL 60-631 Poznań, Poland.
Pol J Pharmacol. 2003 Jan-Feb;55(1):97-102.
The study attempts to evaluate whether it is possible to determine time and level of exposure of rats to tobacco smoke based on nicotine and cotinine content in urine. The animals were exposed to tobacco smoke by inhalation in a specially designed experimental chambers. The exposure to three different tobacco smoke levels (500, 1000 and 1500 mg CO/m3 of air) lasted 6 h per day, for one, three and five days. Nicotine and cotinine concentrations were measured in daily urine using high performance liquid chromatography procedure developed by the authors. It has been shown that cotinine but not nicotine can be used as a biomarker of time and extent of exposure to tobacco smoke.
该研究试图评估是否有可能根据尿液中尼古丁和可替宁的含量来确定大鼠接触烟草烟雾的时间和程度。通过在专门设计的实验舱中吸入烟草烟雾来使动物接触。暴露于三种不同烟草烟雾水平（空气中500、1000和1500毫克一氧化碳/立方米），每天持续6小时，分别持续1天、3天和5天。使用作者开发的高效液相色谱法测量每日尿液中的尼古丁和可替宁浓度。结果表明，可替宁而非尼古丁可作为接触烟草烟雾时间和程度的生物标志物。
