McNiel Elizabeth A, Carmella Steven G, Heath Lori A, Bliss Robin L, Le Ky-Anh, Hecht Stephen S
University of Minnesota Cancer Center, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.
Am J Vet Res. 2007 Apr;68(4):349-53. doi: 10.2460/ajvr.68.4.349.
To evaluate the use of urinary biomarkers to assess exposure of cats to environmental tobacco smoke (ETS).
61 healthy client-owned cats (19 from households in which smoking was reported and 42 from households in which there was no smoking).
Urine samples were obtained from each cat and assayed for total nicotine (nicotine plus nicotine glucuronide) and total cotinine (cotinine plus cotinine glucuronide) content by use of gas chromatography-mass spectrometry. In addition, total urinary content of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), a major metabolite of the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, was measured by use of gas chromatography with nitrosamine-selective detection.
Cats from households in which smoking was reported had significantly higher concentrations of total nicotine (70.4 ng/mL), total cotinine (8.53 ng/mL), and total NNAL (0.0562 pmol/mL) in urine, compared with concentrations for cats that lived in households in which there was no smoking (4.89 ng/mL, 0.74 ng/mL, and 0.0182 pmol/mL, respectively).
Analysis of these data provided biochemical evidence of exposure to ETS and uptake of tobacco-specific carcinogens by cats that live in households with smokers. Biomarkers could facilitate investigation of the health effects of ETS in cats and other species.
评估使用尿液生物标志物来评估猫暴露于环境烟草烟雾（ETS）的情况。
61只健康的由客户拥有的猫（19只来自报告有吸烟情况的家庭，42只来自无吸烟情况的家庭）。
从每只猫获取尿液样本，使用气相色谱 - 质谱法测定总尼古丁（尼古丁加尼古丁葡糖醛酸）和总可替宁（可替宁加可替宁葡糖醛酸）含量。此外，使用具有亚硝胺选择性检测的气相色谱法测量烟草特异性亚硝胺4 -（甲基亚硝胺）-1 -（3 -吡啶基）-1 -丁酮的主要代谢物4 -（甲基亚硝胺）-1 -（3 -吡啶基）-1 -丁醇（NNAL）的总尿液含量。
与生活在无吸烟家庭的猫相比，报告有吸烟情况家庭的猫尿液中总尼古丁（70.ng/mL）、总可替宁（8.53 ng/mL）和总NNAL（0.0562 pmol/mL）浓度显著更高（分别为4.89 ng/mL、0.74 ng/mL和0.0182 pmol/mL）。
对这些数据的分析提供了生活在有吸烟者家庭中的猫暴露于ETS并摄取烟草特异性致癌物的生化证据。生物标志物有助于调查ETS对猫和其他物种的健康影响。
