Mumm Roland, Schrank Kai, Wegener Robert, Schulz Stefan, Hilker Monika
Institute of Biology, Freie Universität Berlin, Haderslebener Str. 9, 12163 Berlin, Germany.
J Chem Ecol. 2003 May;29(5):1235-52. doi: 10.1023/a:1023841909199.
Gas chromatography-mass spectrometry analyses of the headspace volatiles of Scots pine (Pinus sylvestris) induced by egg deposition of the sawfly Diprion pini were conducted. The odor blend of systemically oviposition-induced pine twigs. attractive for the eulophid egg parasitoid Chrysonotomyia ruforum, was compared to volatiles released by damaged pine twigs (control) that are not attractive for the parasitoid. The mechanical damage inflicted to the control twigs mimicked the damage by a sawfly female prior to egg deposition. The odor blend released by oviposition-induced pine twigs consisted of numerous mono- and sesquiterpenes, which all were also present in the headspace of the artificially damaged control twigs. A quantitative comparison of the volatiles from oviposition-induced twigs and controls revealed that only the amounts of (E)-beta-farnesene were significantly higher in the volatile blend of the oviposition-induced twigs. Volatiles from pine twigs treated with jasmonic acid (JA) also attract the egg parasitoid. No qualitative differences were detected when comparing the composition of the headspace of JA-treated pine twigs with the volatile blend of untreated control twigs. JA-treated pine twigs released significantly higher amounts of (E )-beta-farnesene. However, the JA treatment induced a significant increase of the amount of further terpenoid components. The release of terpenoids by pine after wounding, egg deposition, and JA treatment is discussed with special respect to (E)-beta-farnesene.
对由松树叶蜂（Diprion pini）产卵诱导的欧洲赤松（Pinus sylvestris）顶空挥发物进行了气相色谱 - 质谱分析。将系统产卵诱导的松树嫩枝的气味混合物（对姬小蜂科卵寄生蜂红腹金小蜂（Chrysonotomyia ruforum）有吸引力）与未受损伤的松树嫩枝（对照，对寄生蜂没有吸引力）释放的挥发物进行了比较。对照嫩枝遭受的机械损伤模拟了叶蜂雌虫在产卵前造成的损伤。产卵诱导的松树嫩枝释放的气味混合物由多种单萜和倍半萜组成，这些物质在人工损伤的对照嫩枝的顶空中也都存在。对产卵诱导嫩枝和对照挥发物的定量比较表明，只有（E）-β-法尼烯的含量在产卵诱导嫩枝的挥发物混合物中显著更高。用茉莉酸（JA）处理的松树嫩枝的挥发物也吸引卵寄生蜂。将用JA处理的松树嫩枝的顶空成分与未处理的对照嫩枝的挥发物混合物进行比较时，未检测到定性差异。用JA处理的松树嫩枝释放出显著更多的（E）-β-法尼烯。然而，JA处理导致其他萜类成分的含量显著增加。本文特别针对（E）-β-法尼烯讨论了松树在受伤、产卵和JA处理后萜类化合物的释放情况。
