L-NAME对链脲佐菌素诱导的糖尿病大鼠一氧化氮合酶的抑制作用：对氧化应激的影响

Nitric oxide synthase inhibition by L-NAME in streptozotocin induced diabetic rats: impacts on oxidative stress.

作者信息

Seven Arzu, Güzel Savas, Seymen Oktay, Civelek Sabiha, Bolayirli Murat, Yiğit Günnur, Burçak Gülden

机构信息

Department of Biochemistry, Cerrahpaşa Medical Faculty, Istanbul University, Istanbul, Turkey.

出版信息

Tohoku J Exp Med. 2003 Apr;199(4):205-10. doi: 10.1620/tjem.199.205.

DOI:10.1620/tjem.199.205

PMID:12857060

Abstract

The effects of nitric oxide synthase (NOS) inhibition by Nw-nitro-L-arginine methyl ester (L-NAME) administration on oxidative stress parameters were investigated in streptozotocin (STZ) induced diabetic rats. Lipid peroxidation as reflected by thiobarbituric acid reactive substances (TBARS) was insignificantly higher in diabetic rats. Plasma NO2+NO3 values (p < 0.05) and erythrocyte CuZn superoxide dismutase (CuZn SOD) and glutathione peroxidase (GSH Px) activities were significantly higher (p < 0.01, p < 0.001, respectively) in diabetic rats. L-NAME administration to diabetic rats caused significantly lower CuZn SOD and GSH Px activities (p < 0.01) and NO2+NO3 values (p < 0.001), whereas a significantly higher GSH level (p < 0.01). TBARS/GSH ratio was significantly higher in diabetic rats than controls (p < 0.05) and significantly lower in L-NAME administered diabetic rats than diabetic rats (p < 0.05). This experimental study highlightens the importance of NOS inhibition by L-NAME in the attenuation of oxidative stress in STZ diabetic rats.

摘要

研究了通过给予Nω-硝基-L-精氨酸甲酯（L-NAME）抑制一氧化氮合酶（NOS）对链脲佐菌素（STZ）诱导的糖尿病大鼠氧化应激参数的影响。用硫代巴比妥酸反应性物质（TBARS）反映的脂质过氧化在糖尿病大鼠中略高，但无统计学意义。糖尿病大鼠的血浆NO2+NO3值（p<0.05）以及红细胞铜锌超氧化物歧化酶（CuZn SOD）和谷胱甘肽过氧化物酶（GSH Px）活性显著更高（分别为p<0.01，p<0.001）。给糖尿病大鼠施用L-NAME导致CuZn SOD和GSH Px活性显著降低（p<0.01）以及NO2+NO3值显著降低（p<0.001），而谷胱甘肽（GSH）水平显著升高（p<0.01）。糖尿病大鼠的TBARS/GSH比值显著高于对照组（p<0.05），而施用L-NAME的糖尿病大鼠的该比值显著低于糖尿病大鼠（p<0.05）。本实验研究强调了L-NAME抑制NOS在减轻STZ糖尿病大鼠氧化应激中的重要性。