Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Rod. Celso Garcia Cid Km480 PR445, Cx Postal 10.011, Londrina, Paraná, CEP 86057-970, Brazil.
Departamento de Ciências Farmacêuticas, Centro de Ciências de Saúde, Universidade Estadual de Londrina, Londrina, Brazil.
Inflamm Res. 2018 Dec;67(11-12):997-1012. doi: 10.1007/s00011-018-1195-y. Epub 2018 Oct 28.
To evaluate the effect and mechanisms of naringenin in TiO-induced chronic arthritis in mice, a model resembling prosthesis and implant inflammation.
Flavonoids are antioxidant and anti-inflammatory molecules with important anti-inflammatory effect. Mice were daily treated with the flavonoid naringenin (16.7-150 mg/kg, orally) for 30 days starting 24 h after intra-articular knee injection of 3 mg of TiO.
TiO-induced arthritis resembles cases of aseptic inflammation induced by prosthesis and/or implants. Mice were stimulated with 3 mg of TiO and after 24 h mice started to be treated with naringenin. The disease phenotype, treatment toxicity, histopathological damage, oxidative stress, cytokine expression and NFκB were evaluated after 30 days of treatment.
Naringenin inhibited TiO-induced mechanical hyperalgesia (96%), edema (77%) and leukocyte recruitment (74%) without inducing toxicity. Naringenin inhibited histopathological index (HE, 49%), cartilage damage (Toluidine blue tibial staining 49%, and proteoglycan 98%), and bone resorption (TRAP-stained 73%). These effects were accompanied by inhibition of oxidative stress (gp91 93%, NBT 83%, and TBARS 41%) cytokine mRNA expression (IL-33 82%, TNFα 76%, pro-IL-1β 100%, and IL-6 61%), and NFκB activation (100%).
Naringenin ameliorates TiO-induced chronic arthritis inducing analgesic and anti-inflammatory responses with improvement in the histopathological index, cartilage damage, and bone resorption.
评估柚皮素在 TiO 诱导的慢性关节炎中的作用和机制，该模型类似于假体和植入物炎症。
类黄酮是具有重要抗炎作用的抗氧化和抗炎分子。在关节内注射 3mg TiO 后 24 小时，每天用黄酮柚皮素（16.7-150mg/kg，口服）治疗小鼠 30 天。
TiO 诱导的关节炎类似于由假体和/或植入物引起的无菌性炎症病例。用 3mg TiO 刺激小鼠，24 小时后开始用柚皮素治疗小鼠。在治疗 30 天后评估疾病表型、治疗毒性、组织病理学损伤、氧化应激、细胞因子表达和 NFκB。
柚皮素抑制 TiO 诱导的机械性痛觉过敏（96%）、水肿（77%）和白细胞募集（74%），而无毒性。柚皮素抑制组织病理学指数（HE，49%）、软骨损伤（甲苯胺蓝胫骨染色 49%，和糖胺聚糖 98%）和骨吸收（TRAP 染色 73%）。这些作用伴随着氧化应激抑制（gp91 93%，NBT 83%，和 TBARS 41%）细胞因子 mRNA 表达（IL-33 82%，TNFα 76%，pro-IL-1β 100%，和 IL-6 61%）和 NFκB 激活（100%）。
柚皮素改善了 TiO 诱导的慢性关节炎，引起镇痛和抗炎反应，改善了组织病理学指数、软骨损伤和骨吸收。
