Bányai Krisztián, Gentsch Jon R, Griffin Dixie D, Holmes Jennifer L, Glass Roger I, Szücs György
Regional Laboratory of Virology, Baranya County Institute of State Public Health Service, Pécs, Hungary.
J Med Virol. 2003 Sep;71(1):124-34. doi: 10.1002/jmv.10462.
Rotavirus serotype G6 has been demonstrated to be a rare cause of gastroenteritis in man. To date, only a few well characterized strains have been described from Italy, Australia, and the United States. Nucleotide sequencing of G6 VP7 genes shows that these strains belong to two distinct G6 lineages, one for strains of serotype P11[14],G6 (PA169-like strains) and one for strains of serotype P3[9],G6 (PA151-like strains). In this study, we sequenced the VP7 genes and VP8* gene fragments of human rotavirus G6 strains detected in Hungary. Phylogenetic analysis demonstrated that the VP7 genes of Hungarian G6 strains fell into three lineages, represented by a single PA169-like strain, three PA151-like strains, and two novel G6 strains, respectively. The amino acid sequence identity of VP7 was 97.2-100% within each lineage and 92-93.9% between any two lineages. The sequence analysis of VP8* revealed that the single PA169-like Hungarian G6 strain belonged to genotype P[14] and was phylogenetically closely related to P11[14],G6 strains characterized previously. In contrast, the VP8* of PA151-like Hungarian G6 strains clustered in accordance with their VP7 genes representing genetically distinguishable variants of genotype P[9]. This finding raises the possibility that Hungarian genotype P[9],G6 strains might have been generated through independent reassortment events. Serotype G6-specific primers for each human G6 lineage were also developed. The use of these primers in reverse-transcription polymerase chain reaction genotyping may help determine the epidemiological role of G6 strains in humans.
轮状病毒G6血清型已被证明是人类胃肠炎的罕见病因。迄今为止，仅从意大利、澳大利亚和美国描述了少数特征明确的毒株。G6 VP7基因的核苷酸测序表明，这些毒株属于两个不同的G6谱系，一个是血清型P11[14]、G6（PA169样毒株）的毒株，另一个是血清型P3[9]、G6（PA151样毒株）的毒株。在本研究中，我们对在匈牙利检测到的人轮状病毒G6毒株的VP7基因和VP8基因片段进行了测序。系统发育分析表明，匈牙利G6毒株的VP7基因分为三个谱系，分别由一个单一的PA169样毒株、三个PA151样毒株和两个新型G6毒株代表。每个谱系内VP7的氨基酸序列同一性为97.2 - 100%，任何两个谱系之间为92 - 93.9%。VP8的序列分析表明，单一的PA169样匈牙利G6毒株属于基因型P[14]，并且在系统发育上与先前鉴定的P11[14]、G6毒株密切相关。相比之下，PA151样匈牙利G6毒株的VP8*根据其VP7基因聚类，代表基因型P[9]的遗传可区分变体。这一发现增加了匈牙利基因型P[9]、G6毒株可能通过独立重配事件产生的可能性。还开发了针对每个人G6谱系的G6血清型特异性引物。在逆转录聚合酶链反应基因分型中使用这些引物可能有助于确定G6毒株在人类中的流行病学作用。
