Shin Jihye, Park Gyu-Nam, Choe SeEun, Cha Ra Mi, Kim Ki-Sun, An Byung-Hyun, Kim Song Yi, Moon Soo Hyun, Hyun Bang-Hun, An Dong-Jun
Virus Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Republic of Korea.
College of Veterinary Medicine, Seoul University, Seoul 08826, Republic of Korea.
Animals (Basel). 2023 Jul 14;13(14):2314. doi: 10.3390/ani13142314.
This study aimed to investigate the genetic diversity of G- and P-type bovine RVAs (BoRVAs) prevalent in Vietnam. Between 2017 and 2018, the prevalence of BoRVAs detected in diarrhea samples from 8 regions was as low as 1.9% (11/582). The prevalence of the G-type was 45.5% for G6 and 18.2% for G10; however, 36.3% remain unidentified. Interestingly, all BoRVAs were investigated as P[11], and there was no diversity within this P-type. Geographically, the G6 and G10 types were not identified in any specific area; rather, they occurred in both Northern and Southern Vietnam. G6P[11] and G10P[11], which are combined G- and P-types, were identified in 71.4% and 28.6% of BoRVA-positive samples, respectively. Phylogenetic tree analysis revealed that the G6-type detected in Vietnamese cows is similar to strains derived from China, Japan, and Korea, whereas the G10 type is closely related to the Chinese strain. In addition, the P11 strain detected in Vietnamese cows is similar to the Spanish and Chinese strains. The BoRVA-positive rate was higher in cows aged less than 2 months (3.2%, 3/94) than in those aged 2 months or more (1.6%, 8/488). In summary, we detected the presence of G6P11 and G10P11 BoVRAs on Vietnamese cow farms, and found that they were more predominant in young calves than in older cows.
本研究旨在调查越南流行的G型和P型牛轮状病毒A（BoRVA）的遗传多样性。2017年至2018年期间，在8个地区腹泻样本中检测到的BoRVA患病率低至1.9%（11/582）。G型中，G6型的患病率为45.5%，G10型为18.2%；然而，36.3%仍未鉴定。有趣的是，所有BoRVA均被检测为P[11]，且该P型内无多样性。在地理上，未在任何特定区域鉴定出G6型和G10型；相反，它们在越南北部和南部均有出现。在BoRVA阳性样本中，G6P[11]和G10P[11]（G型和P型组合）分别占71.4%和28.6%。系统发育树分析显示，在越南奶牛中检测到的G6型与源自中国、日本和韩国的毒株相似，而G10型与中国毒株密切相关。此外，在越南奶牛中检测到的P11毒株与西班牙和中国的毒株相似。小于2月龄奶牛的BoRVA阳性率（3.2%，3/94）高于2月龄及以上奶牛（1.6%，8/488）。总之，我们在越南奶牛场检测到了G6P11和G10P11 BoRVA的存在，并发现它们在幼龄犊牛中比在成年奶牛中更占优势。
