抗环瓜氨酸肽抗体在类风湿关节炎患者中的诊断价值。

Diagnostic value of anti-cyclic citrullinated Peptide antibody in patients with rheumatoid arthritis.

作者信息

Zeng Xiaofeng, Ai Maixing, Tian Xinping, Gan Xiaodan, Shi Yanping, Song Qinfang, Tang Fulin

机构信息

Department of Rheumatology, Chinese Academy of Medical Science, Peking Union Medical College Hospital, Beijing, China.

出版信息

J Rheumatol. 2003 Jul;30(7):1451-5.

DOI:

PMID:12858440

Abstract

OBJECTIVE

To explore the diagnostic value of anti-cyclic citrullinated peptide antibody (anti-CCP) detected by ELISA in patients with rheumatoid arthritis (RA).

METHODS

The synthesized cyclic citrullinated peptide was used as substrate for ELISA. Anti-CCP antibody was detected by ELISA in 191 patients with RA, 132 with rheumatic diseases other than RA, and 98 with nonrheumatic diseases. The antiperinuclear factor (APF), anti-keratin antibody (AKA), rheumatoid factor (RF), and HLA-DR4 gene complex were also tested in each RA patient. The results of these tests were compared with anti-CCP antibody to examine the correlation between them.

RESULTS

Ninety (47.1%) patients with RA, 4 (3.0%) with other rheumatic diseases, and 2 (2.0%) with nonrheumatic diseases were found to be anti-CCP antibody positive by ELISA. The sensitivity of anti-CCP antibody was 47.1%, with a high specificity (97.4%) in RA. Anti-CCP antibody correlated with APF, AKA, RF, and HLA-DR4 gene complex.

CONCLUSION

A new modified anti-CCP antibody test had a moderate sensitivity (47.1%) but a high specificity (97.4%) in patients with RA and was found as a valuable supplement to diagnosis of RA. Anti-CCP correlated with APF, AKA, RF, and HLA-DR4 gene complex, but did not completely overlap with them. Anti-CCP antibody could be regarded as a new diagnostic marker for RA.

摘要

目的

探讨酶联免疫吸附测定（ELISA）检测抗环瓜氨酸肽抗体（抗CCP）在类风湿关节炎（RA）患者中的诊断价值。

方法

以合成的环瓜氨酸肽为ELISA底物。采用ELISA法检测191例RA患者、132例非RA风湿性疾病患者和98例非风湿性疾病患者的抗CCP抗体。对每位RA患者还检测了抗核周因子（APF）、抗角蛋白抗体（AKA）、类风湿因子（RF）和HLA - DR4基因复合体。将这些检测结果与抗CCP抗体进行比较，以研究它们之间的相关性。