Axelsson L, Lindstad G, Naterstad K
MATFORSK, Norwegian Food Research Institute, Osloveien, Norway.
Lett Appl Microbiol. 2003;37(2):115-20. doi: 10.1046/j.1472-765x.2003.01360.x.
To develop an inducible gene expression system for Lactobacillus sakei, based on the regulatory system of sakacin A production.
A Lactobacillus/Escherichia coli shuttle vector; pKRV3, was constructed including the signal transducing system genes of the bacteriocin sakacin A. The gusA gene fused to PsapA promoter, cloned in this vector allowed for inducible beta-glucuronidase expression in L. sakei and L. plantarum following the addition of the sakacin A inducing peptide. PsapA appeared to be a strong and tightly controlled promoter when compared with known promoters.
The pKRV3 system can be used as an inducible gene expression system in lactobacilli.
A novel, inducible gene expression system has been developed for lactic acid bacteria relevant in food fermentations.
