Capucchio M T, Sanna E, Sanna M P, Farigu S, Minelli R, Guarda F
Dipartimento di Patologia Animale, Facoltà di Medicina Veterinaria, Università di Torino, Via Leonardo Da Vinci 44, 10095 Grugliasco, Turin, Italy.
J Comp Pathol. 2003 Jul;129(1):37-43. doi: 10.1016/s0021-9975(02)00158-5.
Maedi-visna is a systemic disease of sheep caused by a lentivirus, maedi-visna virus (MVV), which mainly affects the lungs and central nervous system but may also affect the mammary glands, joints and other tissues. The aim of the present study was to determine whether the third eyelid was affected in cases of systemic infection. Third eyelid and lung samples from sheep naturally infected with maedi were used. Total DNA was extracted from paraffin-wax-embedded tissues, and a nested polymerase chain reaction (PCR) was performed to amplify MVV proviral DNA. The samples were also tested by in-situ PCR and immunohistochemical methods specific for the detection of MVV proviral DNA and p25, respectively. All sheep showed moderate to severe chronic lymphoproliferative inflammation in the third eyelids. Products of the expected size were obtained by PCR from both lung and third eyelid tissue. In the nictitating membrane, MVV proviral DNA was detected in situ within macrophages, and glandular, ductal and surface epithelia. Immunohistochemistry demonstrated that the infection was productive. Taken together, these results indicate that the third eyelid may represent a target for natural MVV infection and may play a role in disease transmission.
梅迪-维斯纳病是一种由慢病毒——梅迪-维斯纳病毒（MVV）引起的绵羊全身性疾病，该病毒主要影响肺部和中枢神经系统，但也可能影响乳腺、关节和其他组织。本研究的目的是确定在全身性感染病例中第三眼睑是否受到影响。使用了来自自然感染梅迪病的绵羊的第三眼睑和肺样本。从石蜡包埋组织中提取总DNA，并进行巢式聚合酶链反应（PCR）以扩增MVV前病毒DNA。还分别通过原位PCR和针对检测MVV前病毒DNA和p25的免疫组织化学方法对样本进行检测。所有绵羊的第三眼睑均表现出中度至重度慢性淋巴细胞增生性炎症。通过PCR从肺和第三眼睑组织中均获得了预期大小的产物。在瞬膜中，在巨噬细胞以及腺上皮、导管上皮和表面上皮细胞内原位检测到MVV前病毒DNA。免疫组织化学表明感染具有增殖性。综上所述，这些结果表明第三眼睑可能是MVV自然感染的靶器官，并且可能在疾病传播中起作用。
