LL-37/hCAP-18基因在人白血病细胞中的表达。

Expression of LL-37/hCAP-18 gene in human leukemia cells.

作者信息

Yang Ying-Hua, Zheng Guo-Guang, Li Ge, Zhang Bin, Song Yu-Hua, Wu Ke-Fu

机构信息

National Laboratory of Experimental Hematology, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, PR China.

出版信息

Leuk Res. 2003 Oct;27(10):947-50. doi: 10.1016/s0145-2126(03)00020-1.

DOI:10.1016/s0145-2126(03)00020-1

PMID:12860015

Abstract

LL-37/hCAP-18 is an important part of host defense. Several diseases in human are characterized by impairment in the function of LL-37/hCAP-18 peptide. We examined the expression of LL-37/hCAP-18 in a panel of hematopoietic cell lines representing multiple cell lineages. LL-37/hCAP-18 expression at mRNA level was detected and varied among six of nine cell lines. The level of Raji cells was about eight folds higher than that of Ramos cells. However, only two cell lines, J6-1 and U937, expressed protein products. We also investigated LL-37/hCAP-18 protein in nine leukemia and three idiopathic thrombocytopenic purpura (ITP) patients via immunocytochemical staining. The rate of LL-37/hCAP-18 positive cells ranged from 60 (ITP) to 0.5% (M5). These data suggested that the low translation efficiency of LL-37/hCAP-18 expresses in some leukemia cells might be one of the reasons that leukemia patients were susceptible to infection.

摘要

LL-37/hCAP-18是宿主防御的重要组成部分。人类的几种疾病的特征是LL-37/hCAP-18肽功能受损。我们检测了一组代表多个细胞谱系的造血细胞系中LL-37/hCAP-18的表达。在mRNA水平检测到LL-37/hCAP-18的表达，且在九个细胞系中的六个细胞系中有所不同。Raji细胞的水平比Ramos细胞高约八倍。然而，只有两个细胞系J6-1和U937表达蛋白产物。我们还通过免疫细胞化学染色研究了9例白血病患者和3例特发性血小板减少性紫癜（ITP）患者中的LL-37/hCAP-18蛋白。LL-37/hCAP-18阳性细胞的比例范围为60%（ITP）至0.5%（M5）。这些数据表明，LL-37/hCAP-18在某些白血病细胞中的低翻译效率可能是白血病患者易感染的原因之一。