Sonoda Takanori, Kobayashi Hiroaki, Kaku Tsunehisa, Hirakawa Toshio, Nakano Hitoo
Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Cancer Lett. 2003 Jul 10;196(2):229-37. doi: 10.1016/s0304-3835(03)00216-7.
We examined the expression of four angiogenesis factors (vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), platelet-derived endothelial cell growth factor (PD-ECGF) and basic fibroblast growth factor (bFGF)) in five human ovarian cancer cell lines by Northern blot analysis and immunohistochemical staining. The cancer cells were grown as a subconfluent monolayer culture, a multicellular aggregate (spheroid) in a three-dimensional culture, and a nude mouse-transplanted subcutaneous tumor in order to simulate the cellular conditions of ovarian cancers in peritonitis carcinomatosa, i.e. floating single tumor cells, multicellular aggregates and peritoneally implanted tumors. In each cell line, the expression of VEGF was detected in a monolayer culture and obviously enhanced in a three-dimensional culture. IL-8 was expressed in two of five cultured cell lines, but neither PD-ECGF nor bFGF was detected. Each cell line-derived transplanted tumor expressed immunohistochemical products of the four angiogenesis factors examined. These observations were confirmed by surgical specimens and suggested that ovarian cancer cells expressed different kinds and/or doses of angiogenesis factors depending on the form of the changed tumor cells during peritoneal implant formation.
我们通过Northern印迹分析和免疫组织化学染色，检测了五种人卵巢癌细胞系中四种血管生成因子（血管内皮生长因子（VEGF）、白细胞介素-8（IL-8）、血小板衍生的内皮细胞生长因子（PD-ECGF）和碱性成纤维细胞生长因子（bFGF））的表达。为了模拟癌性腹膜炎中卵巢癌的细胞状况，即漂浮的单个肿瘤细胞、多细胞聚集体和腹膜植入肿瘤，将癌细胞培养成亚汇合单层培养物、三维培养中的多细胞聚集体（球体）以及裸鼠移植的皮下肿瘤。在每种细胞系中，VEGF的表达在单层培养中被检测到，并且在三维培养中明显增强。IL-8在五种培养的细胞系中的两种中表达，但未检测到PD-ECGF和bFGF。每种细胞系来源的移植肿瘤均表达所检测的四种血管生成因子的免疫组织化学产物。这些观察结果在手术标本中得到证实，并表明卵巢癌细胞根据腹膜植入形成过程中肿瘤细胞变化的形式表达不同种类和/或剂量的血管生成因子。
