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纤维溶解丁酸弧菌H10b菌株产生的细胞外多糖的结构研究。

Structural studies of the extracellular polysaccharide produced by Butyrivibrio fibrisolvens strain H10b.

作者信息

Andersson Lars, Cotta Michael A, Kenne Lennart

机构信息

Department of Chemistry, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden.

出版信息

Carbohydr Res. 2003 Jul 22;338(15):1571-9. doi: 10.1016/s0008-6215(03)00204-0.

Abstract

The extracellular polysaccharide produced by Butyrivibrio fibrisolvens strain H10b, when grown under strictly anaerobic conditions with glucose as carbohydrate source, has been studied by chemical and spectroscopic techniques. The results demonstrate that the polysaccharide consists of hexasaccharide repeating units with the following structure: [structure: see text] The isolated polysaccharide was found to be approximately 65% acetylated at O-2 of the 3-O-[(S)-1-carboxyethyl]-beta-D-Glcp residue. The absolute configuration of the 1-carboxyethyl groups was determined by circular dichroism.

摘要

在以葡萄糖作为碳水化合物来源、严格厌氧条件下培养时,纤维溶解丁酸弧菌H10b菌株产生的细胞外多糖已通过化学和光谱技术进行了研究。结果表明,该多糖由具有以下结构的六糖重复单元组成:[结构:见正文]。发现分离出的多糖在3-O-[(S)-1-羧乙基]-β-D-葡萄糖残基的O-2处约65%被乙酰化。通过圆二色性确定了1-羧乙基基团的绝对构型。

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