Graf Martin R, Prins Robert M, Poulsen Gail A, Merchant Randall E
Department of Anatomy and Neurobiology, Medical College of Virginia, Virginia Commonwealth University, P.O. Box 980709 MCV Station, Richmond, VA 23298-0709, USA.
J Neuroimmunol. 2003 Jul;140(1-2):49-60. doi: 10.1016/s0165-5728(03)00167-x.
Rat T9.F glioma cells were transduced with the interleukin (IL)-2 gene. Clone T9.F/IL2/#12 secreted a high level of IL-2 (15 ng/10(6) cells/48 h). Enhanced tumor progression and reduced survival was observed when T9.F/IL2/#12 cells were implanted intracranially. Subcutaneous injection of T9.F/IL2/#12 cells induced a palpable nodule, which regressed in approximately 15 days, resulting in tumor-specific protection. Lymphocytes from T9.F/IL2/#12 primed rats specifically respond to T9.F antigens but lacked cytotoxicity towards T9.F cells. Intracranial T9.F/IL2/#12 tumors were markedly infiltrated by CD4(+) and CD8(+) T cells, natural killer (NK)-T cells and myeloid progenitor cells, whereas subcutaneous T9.F/IL2/#12 tumors contained an elevated level of NK cells.
用白细胞介素（IL）-2基因转导大鼠T9.F胶质瘤细胞。克隆T9.F/IL2/#12分泌高水平的IL-2（15 ng/10(6)细胞/48小时）。当将T9.F/IL2/#12细胞颅内植入时，观察到肿瘤进展加快和生存期缩短。皮下注射T9.F/IL2/#12细胞诱导出一个可触及的结节，该结节在大约15天内消退，产生肿瘤特异性保护作用。来自T9.F/IL2/#12致敏大鼠的淋巴细胞对T9.F抗原产生特异性反应，但对T9.F细胞缺乏细胞毒性。颅内T9.F/IL2/#12肿瘤被CD4(+)和CD8(+) T细胞、自然杀伤（NK）-T细胞和髓系祖细胞显著浸润，而皮下T9.F/IL2/#12肿瘤含有升高水平的NK细胞。
