Gresl Theresa A, Colman Ricki J, Havighurst Thomas C, Allison David B, Schoeller Dale A, Kemnitz Joseph W
Wisconsin National Primate Research Center and Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, 53715, USA.
J Gerontol A Biol Sci Med Sci. 2003 Jul;58(7):598-610. doi: 10.1093/gerona/58.7.b598.
We examined the effects of dietary restriction (DR) and age on ss-cell function and peripheral insulin sensitivity in rhesus monkeys. A semipurified diet was provided either ad libitum for approximately 8 hours/day to controls (C) or as approximately 70% of baseline intake to restricted (R) animals for 10 years. The minimal model of C-peptide secretion and kinetics and the labeled 2-compartment minimal model of glucose kinetics were identified using plasma glucose, C-peptide, and insulin concentrations during an intravenous glucose tolerance test. R monkeys had less body fat, lower basal ss-cell sensitivity to glucose (Ø(b)), greater insulin sensitivity, and lower first-phase plasma insulin response. DR did not significantly affect first-phase and second-phase ss-cell sensitivity to glucose. Indices of body fatness were highly predictive of the effect of DR on Ø(b), fasting insulin concentration and insulin responses to glucose. Enhanced peripheral insulin sensitivity among R monkeys was strongly correlated with lower Ø(b).
我们研究了饮食限制（DR）和年龄对恒河猴胰岛β细胞功能及外周胰岛素敏感性的影响。为对照组（C）提供半纯化饮食，使其每天随意进食约8小时；为限制饮食组（R）的动物提供约为基线摄入量70%的食物，持续10年。在静脉葡萄糖耐量试验期间，利用血浆葡萄糖、C肽和胰岛素浓度，确定了C肽分泌和动力学的最小模型以及葡萄糖动力学的标记双室最小模型。R组猴子体脂较少，基础胰岛β细胞对葡萄糖的敏感性（Ø(b)）较低，胰岛素敏感性较高，且第一相血浆胰岛素反应较低。饮食限制并未显著影响第一相和第二相对葡萄糖的胰岛β细胞敏感性。体脂指数高度预测了饮食限制对Ø(b)、空腹胰岛素浓度及胰岛素对葡萄糖反应的影响。R组猴子外周胰岛素敏感性增强与较低的Ø(b)密切相关。
