VanBogelen R A, Sankar P, Clark R L, Bogan J A, Neidhardt F C
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor.
Electrophoresis. 1992 Dec;13(12):1014-54. doi: 10.1002/elps.11501301203.
The gene-protein database of Escherichia coli is both an index relating a gene to its protein product on two-dimensional gels, and a catalog of information about the function, regulation, and genetics of individual proteins obtained from two-dimensional gel analysis or collated from the literature. Edition 5 has 102 new entries--a 15% increase in the number of annotated two-dimensional gel spots. The large increase in this edition was accomplished in part by the use of a new method for expression analysis of ordered segments of the E. coli genome, which has resulted in linking 50 gel spots to their genes (or open reading frames) and another 45 to specific regions of the chromosome awaiting the availability of DNA sequence information. Communication of information from the scientific community resulted in additional identifications and regulatory information. To increase accessibility of the database it has been placed in the repository at the National Center for Biotechnology Information (NCBI) at the National Library of Medicine under the name ECO2DBASE. It will be updated twice yearly. This edition of the gene-protein database is estimated to contain entries for one-sixth of the protein-encoding genes of E. coli.
大肠杆菌基因 - 蛋白质数据库既是一个将基因与其在二维凝胶上的蛋白质产物相关联的索引，也是一份关于从二维凝胶分析中获得或从文献中整理而来的单个蛋白质的功能、调控和遗传学信息的目录。第5版有102个新条目，注释的二维凝胶斑点数量增加了15%。该版本数量大幅增加部分得益于一种用于大肠杆菌基因组有序片段表达分析的新方法，该方法已将50个凝胶斑点与其基因（或开放阅读框）关联起来，另有45个与染色体的特定区域相关联，等待DNA序列信息的获取。科学界提供的信息带来了更多的鉴定结果和调控信息。为了提高数据库的可访问性，它已以ECO2DBASE的名称存入国立医学图书馆的国家生物技术信息中心（NCBI）的储存库中。它将每年更新两次。这个版本的基因 - 蛋白质数据库估计包含大肠杆菌六分之一的蛋白质编码基因的条目。
