Falkner E, Kapeller B, Eberl H, Frick W, Losert U M, Macfelda K
Department of Biomedical Research, University of Vienna, Vienna, Austria.
Int J Artif Organs. 2003 Jun;26(6):514-9. doi: 10.1177/039139880302600610.
For the purpose of tissue regeneration gels of reconstituted basement membrane have been suggested as a vehicle to transfer autologous cells. Results do look promising, but it should be considered that extracellular matrix (ECM) gel (Matrigel) is a soluble extract of Engelbreth-Holm-Swarm (EHS) mouse tumor. Therefore objections arising concerning possible risks complicate clinical use in human subjects. Aim of this study was to determine whether ECM-components of human origin can be used as substitutes for tissue engineering tasks as proposed previously. Proliferation capability and viability of primary rat myocytes and rat myocyte cell lines were determined on days 1, 2, 4 and 8 after inocculation of the cells. Pooled data suggest that an appropriate combination of human ECM and human Collagen Type IV may represent an approach with good prospects.
为了组织再生，有人提出用重组基底膜凝胶作为载体来转移自体细胞。结果看起来很有前景，但应该考虑到细胞外基质（ECM）凝胶（基质胶）是Engelbreth-Holm-Swarm（EHS）小鼠肿瘤的可溶性提取物。因此，关于可能风险的反对意见使在人类受试者中的临床应用变得复杂。本研究的目的是确定是否可以使用人源ECM成分作为先前提出的组织工程任务的替代品。在接种细胞后的第1、2、4和8天，测定原代大鼠心肌细胞和大鼠心肌细胞系的增殖能力和活力。汇总数据表明，人源ECM和人IV型胶原蛋白的适当组合可能是一种前景良好的方法。
